Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells

Seth L. Blumerman, Fei Wang, Carolyn T A Herzig, Cynthia L. Baldwin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1+ γδ T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1+ γδ T cells induced IFN-γ production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1+ γδ T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1+ γδ T cells is reflected by its chemokine receptor expression and suggest WC1+ γδ T cells are unlikely to transit through secondary lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)87-102
Number of pages16
JournalDevelopmental and Comparative Immunology
Issue number1
StatePublished - 2007


  • CCR5
  • CCR7
  • CXCR3
  • CXCR5
  • WC1
  • γδ T cells

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology


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