TY - JOUR
T1 - Molecular cloning, characterization, and expression of a cDNA encoding the '80- to 87-kDa' myristoylated alanine-rich C kinase substrate
T2 - A major cellular substrate for protein kinase C
AU - Stumpo, D. J.
AU - Graff, J. M.
AU - Albert, K. A.
AU - Greengard, P.
AU - Blackshear, P. J.
PY - 1989
Y1 - 1989
N2 - We isolated and sequenced a cDNA clone encoding the bovine '80- to 87-kDa' protein, a major cellular substrate for protein kinase C. An open reading frame of 1005 base pairs predicted a protein of 335 amino acids (M(r), 31,949). Despite this predicted size, the protein migrated on SDS/polyacrylamide gels with an apparent molecular weight of 80-87,000 after expression of the cDNA in cells lacking the protein. It was highly enriched in alanine (28.4 mol %), contained an amino-terminal myristoylation consensus sequence, and included a 25-residue basic domain containing the known protein kinase C phosphorylation sites. Two mRNA species (2.6 and 4.4 kilobases) were mostly highly expressed in brain, spinal cord, spleen, and lung, in parallel with the distribution of immunoreactive protein. Genomic blot analysis indicated the likelihood of a single gene coding for this mRNA. We propose the name myristoylated alanine-rich C kinase substrate (MARCKS) for this protein.
AB - We isolated and sequenced a cDNA clone encoding the bovine '80- to 87-kDa' protein, a major cellular substrate for protein kinase C. An open reading frame of 1005 base pairs predicted a protein of 335 amino acids (M(r), 31,949). Despite this predicted size, the protein migrated on SDS/polyacrylamide gels with an apparent molecular weight of 80-87,000 after expression of the cDNA in cells lacking the protein. It was highly enriched in alanine (28.4 mol %), contained an amino-terminal myristoylation consensus sequence, and included a 25-residue basic domain containing the known protein kinase C phosphorylation sites. Two mRNA species (2.6 and 4.4 kilobases) were mostly highly expressed in brain, spinal cord, spleen, and lung, in parallel with the distribution of immunoreactive protein. Genomic blot analysis indicated the likelihood of a single gene coding for this mRNA. We propose the name myristoylated alanine-rich C kinase substrate (MARCKS) for this protein.
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U2 - 10.1073/pnas.86.11.4012
DO - 10.1073/pnas.86.11.4012
M3 - Article
C2 - 2726763
AN - SCOPUS:0037731790
SN - 0027-8424
VL - 86
SP - 4012
EP - 4016
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -