TY - JOUR
T1 - Molecular-cloning and tissue-specific expression of fructose 6-phosphate 2-kinase:fructose 2,6-bisphosphatase of rat brain
AU - Watanabe, F.
AU - Sakai, A.
AU - Furuya, E.
AU - Uyeda, K.
PY - 1994/1/15
Y1 - 1994/1/15
N2 - A cDNA clone (3591 base pairs) encoding an isozyme of Fructose 6-phosphate, 2-kinase:Fructose 2,6-bisphosphatase was isolated from a rat brain cDNA library. The 5′ sequence of this clone (1241 base pairs) was identical to that of the heart type isozyme cDNA (except for 7 base pair mismatches), but the 3′ nucleotide sequence (2024 base pairs) was completely different. Its deduced amino acid sequence showed that the enzyme lacked a regulatory domain which contained phosphorylation sites for protein kinase A and C. The results of Northern blotting and polymerase chain reaction demonstrated that the mRNA, 7.4 kilobases long, was expressed also in heart, testis, liver, and skeletal muscle.
AB - A cDNA clone (3591 base pairs) encoding an isozyme of Fructose 6-phosphate, 2-kinase:Fructose 2,6-bisphosphatase was isolated from a rat brain cDNA library. The 5′ sequence of this clone (1241 base pairs) was identical to that of the heart type isozyme cDNA (except for 7 base pair mismatches), but the 3′ nucleotide sequence (2024 base pairs) was completely different. Its deduced amino acid sequence showed that the enzyme lacked a regulatory domain which contained phosphorylation sites for protein kinase A and C. The results of Northern blotting and polymerase chain reaction demonstrated that the mRNA, 7.4 kilobases long, was expressed also in heart, testis, liver, and skeletal muscle.
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U2 - 10.1006/bbrc.1994.1047
DO - 10.1006/bbrc.1994.1047
M3 - Article
C2 - 8292038
AN - SCOPUS:0028272252
SN - 0006-291X
VL - 198
SP - 335
EP - 340
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -