TY - JOUR
T1 - MHC Class I gene organization in >1.5-Mb YAC contigs from the H2-M region
AU - Jones, Elsy P.
AU - Xiao, Hong
AU - Schultz, Roger A.
AU - Flaherty, Lorraine
AU - Trachtulec, Zdenek
AU - Vincek, Vladimir
AU - Larin, Zoia
AU - Lehrach, Hans
AU - Lindahl, Kirsten Fischer
PY - 1995/5/1
Y1 - 1995/5/1
N2 - Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single-and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A//M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.
AB - Sixteen yeast artificial chromosome (YAC) clones have been mapped to the H2-M region at the distal end of the mouse major histocompatibility complex (MHC) on chromosome 17. Analysis of the YACs with single-and multicopy probes yielded a proximal contig spanning a minimum of 800 kb and a distal contig of 700 kb. A probe for the conserved fourth exon of MHC class I genes detected 19 restriction fragments, including 6 of the 8 previously characterized H2-M class I genes, in the proximal contig. This contig spans the gap from the M to the T region and includes the T1 gene. By contrast, only two class I genes, M2 and M3, were found in the distal contig. These two genes, which are both expressed, may mark the end of the MHC. The order among nine class I genes and seven other markers was determined in the cloned DNA from the centromere as T1, Tu32A, (M1-M7-M8), Tu32B, B30, M6, M4, M5, Mog, Tu42A//M2, Leh525, M3, Tu42B, where the orientation with respect to the centromere is unknown for M1-M7-M8.
UR - http://www.scopus.com/inward/record.url?scp=0029054724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029054724&partnerID=8YFLogxK
U2 - 10.1006/geno.1995.1006
DO - 10.1006/geno.1995.1006
M3 - Article
C2 - 7665183
AN - SCOPUS:0029054724
SN - 0888-7543
VL - 27
SP - 40
EP - 51
JO - Genomics
JF - Genomics
IS - 1
ER -