Methylene blue promotes quiescence of rat neural progenitor cells

Luokun Xie; Gourav R. Choudhury; Jixian Wang; Yong Park; Ran Liu; Fang Yuan; Chun Li Zhang; Thomas Yorio; Kunlin Jin; Shao Hua Yang

doi:10.3389/fncel.2014.00315

Methylene blue promotes quiescence of rat neural progenitor cells

Luokun Xie, Gourav R. Choudhury, Jixian Wang, Yong Park, Ran Liu, Fang Yuan, Chun Li Zhang, Thomas Yorio, Kunlin Jin, Shao Hua Yang

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5 Scopus citations

Abstract

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

Original language	English (US)
Article number	315
Journal	Frontiers in Cellular Neuroscience
Volume	8
Issue number	OCT
DOIs	https://doi.org/10.3389/fncel.2014.00315
State	Published - Oct 7 2014

Keywords

Methylene blue
Neural progenitor cell
Neurogenesis
Proliferation
Quiescence

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

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10.3389/fncel.2014.00315

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title = "Methylene blue promotes quiescence of rat neural progenitor cells",

abstract = "Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.",

keywords = "Methylene blue, Neural progenitor cell, Neurogenesis, Proliferation, Quiescence",

author = "Luokun Xie and Choudhury, {Gourav R.} and Jixian Wang and Yong Park and Ran Liu and Fang Yuan and Zhang, {Chun Li} and Thomas Yorio and Kunlin Jin and Yang, {Shao Hua}",

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doi = "10.3389/fncel.2014.00315",

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T1 - Methylene blue promotes quiescence of rat neural progenitor cells

AU - Xie, Luokun

AU - Choudhury, Gourav R.

AU - Wang, Jixian

AU - Park, Yong

AU - Liu, Ran

AU - Yuan, Fang

AU - Zhang, Chun Li

AU - Yorio, Thomas

AU - Jin, Kunlin

AU - Yang, Shao Hua

PY - 2014/10/7

Y1 - 2014/10/7

N2 - Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

AB - Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentiation. Consistently, intracerebroventricular infusion of methylene blue significantly inhibited NPC proliferation at the subventricular zone (SVZ). Methylene blue inhibited mTOR signaling along with down-regulation of cyclins in NPCs in vitro and in vivo. In summary, our study indicates that methylene blue may delay NPC senescence through enhancing NPCs quiescence.

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KW - Proliferation

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Methylene blue promotes quiescence of rat neural progenitor cells

Abstract

Keywords

ASJC Scopus subject areas

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