@book{1229076b6515446a9eeda3603b117753,
title = "Metabolic labeling of glycoconjugates with photocrosslinking sugars",
abstract = "Protein-carbohydrate interactions play essential roles in a variety of biological processes. This class of interactions is particularly important in development, immunology, infection, and carcinogenesis. However, the transient nature of glycan-dependent interactions hampers efforts to detect and characterize these complexes. Photocrosslinking is emerging as a powerful tool to discover and study glycan-dependent complexes. Through the use of photocrosslinking groups, UV irradiation can be employed to introduce a covalent bond between two transiently interacting molecules. Here we describe the use of metabolic oligosaccharide engineering to incorporate a photocrosslinkable sugar into cellular glycoconjugates and the use of this photocrosslinker to covalently capture glycan-mediated interactions.",
author = "Yu, {Seok Ho} and Bond, {Michelle R.} and Whitman, {Chad M.} and Kohler, {Jennifer J.}",
year = "2010",
doi = "10.1016/S0076-6879(10)78026-5",
language = "English (US)",
volume = "478",
series = "Methods in Enzymology",
publisher = "Unknown Publisher",
number = "C",
edition = "C",
}