Membrane fission by dynamin: what we know and what we need to know

Bruno Antonny; Christopher Burd; Pietro De Camilli; Elizabeth Chen; Oliver Daumke; Katja Faelber; Marijn Ford; Vadim A. Frolov; Adam Frost; Jenny E. Hinshaw; Tom Kirchhausen; Michael M. Kozlov; Martin Lenz; Harry H. Low; Harvey McMahon; Christien Merrifield; Thomas D. Pollard; Phillip J. Robinson; Aurélien Roux; Sandra L Schmid

doi:10.15252/embj.201694613

Membrane fission by dynamin: what we know and what we need to know

Bruno Antonny, Christopher Burd, Pietro De Camilli, Elizabeth Chen, Oliver Daumke, Katja Faelber, Marijn Ford, Vadim A. Frolov, Adam Frost, Jenny E. Hinshaw, Tom Kirchhausen, Michael M. Kozlov, Martin Lenz, Harry H. Low, Harvey McMahon, Christien Merrifield, Thomas D. Pollard, Phillip J. Robinson, Aurélien Roux, Sandra L Schmid

Research output: Contribution to journal › Review article › peer-review

276 Scopus citations

Abstract

The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.

Original language	English (US)
Pages (from-to)	2270-2284
Number of pages	15
Journal	EMBO Journal
Volume	35
Issue number	21
DOIs	https://doi.org/10.15252/embj.201694613
State	Published - Nov 2 2016

Keywords

GTPase
dynamin
endocytosis
membrane fission
molecular motor

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.15252/embj.201694613

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Antonny, B., Burd, C., De Camilli, P., Chen, E., Daumke, O., Faelber, K., Ford, M., Frolov, V. A., Frost, A., Hinshaw, J. E., Kirchhausen, T., Kozlov, M. M., Lenz, M., Low, H. H., McMahon, H., Merrifield, C., Pollard, T. D., Robinson, P. J., Roux, A., & Schmid, S. L. (2016). Membrane fission by dynamin: what we know and what we need to know. EMBO Journal, 35(21), 2270-2284. https://doi.org/10.15252/embj.201694613

Antonny, B, Burd, C, De Camilli, P, Chen, E, Daumke, O, Faelber, K, Ford, M, Frolov, VA, Frost, A, Hinshaw, JE, Kirchhausen, T, Kozlov, MM, Lenz, M, Low, HH, McMahon, H, Merrifield, C, Pollard, TD, Robinson, PJ, Roux, A & Schmid, SL 2016, 'Membrane fission by dynamin: what we know and what we need to know', EMBO Journal, vol. 35, no. 21, pp. 2270-2284. https://doi.org/10.15252/embj.201694613

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abstract = "The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.",

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AU - Burd, Christopher

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AU - Faelber, Katja

AU - Ford, Marijn

AU - Frolov, Vadim A.

AU - Frost, Adam

AU - Hinshaw, Jenny E.

AU - Kirchhausen, Tom

AU - Kozlov, Michael M.

AU - Lenz, Martin

AU - Low, Harry H.

AU - McMahon, Harvey

AU - Merrifield, Christien

AU - Pollard, Thomas D.

AU - Robinson, Phillip J.

AU - Roux, Aurélien

AU - Schmid, Sandra L

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N2 - The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.

AB - The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.

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Membrane fission by dynamin: what we know and what we need to know

Abstract

Keywords

ASJC Scopus subject areas

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