MEKK1 binds Raf-1 and the ERK2 cascade components

Mahesh Karandikar, Shuichan Xu, Melanie H. Cobb

Research output: Contribution to journalArticlepeer-review

105 Scopus citations


Mitogen-activated protein (MAP) kinase caseades are involed in transmitting signals that are generated at the cell surface into the cytosol and nucleus and consist of three sequentially acting enzymes: a MAP kinase, an upstream MAP/extracellular signal-regulated protein kinase (ERK) kinase (MEK), and a MEK kinase (MEKK). Protein-protein interactions within these cascades provide a mechanism to control the localization and function of the protiens. MEKK1 is implicated in activation of the c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) and ERK1/2 MAP kinase pathways. We showed previously that MEKK1 binds directly to JNK/SAPK. In this study we demonstrate that endogenous MEKK1 binds to endogenous ERK2, MEK1, and another MEKK level kinase, Raf-1, suggesting that it can assemble all three proteins of the ERK2 MAP kinase module.

Original languageEnglish (US)
Pages (from-to)40120-40127
Number of pages8
JournalJournal of Biological Chemistry
Issue number51
StatePublished - Dec 22 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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