TY - JOUR
T1 - Meiotic prophase requires proteolysis of m phase regulators mediated by the meiosis-specific APC/CAma1
AU - Okaz, Elwy
AU - Argüello-Miranda, Orlando
AU - Bogdanova, Aliona
AU - Vinod, P. K.
AU - Lipp, Jesse J.
AU - Markova, Zuzana
AU - Zagoriy, Ievgeniia
AU - Novak, Bela
AU - Zachariae, Wolfgang
N1 - Funding Information:
We thank Angelika Amon, Kirsten Benjamin, Rita Cha, Franz Klein, Michael Knop, Shirleen Roeder, Karin Schmekel, and Wolfgang Seufert for strains and reagents. This work was supported by the Max Planck Society, the Deutsche Forschungsgemeinschaft (SPP1384 grant ZA519/1-1 to W.Z.), a Boehringer Ingelheim Fonds scholarship to J.J.L., and European Commission FP7 (UniCellSys, MitoSys) and OCISB grants to B.N.
PY - 2012/10/26
Y1 - 2012/10/26
N2 - Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.
AB - Whereas proliferating cells enter M phase shortly after DNA replication, the first M phase of meiosis is preceded by an extended prophase in which homologous chromosomes undergo recombination. Exit from prophase I is controlled by the recombination checkpoint (RC), which, in yeast, represses the meiosis-specific transcription factor Ndt80 required for the expression of B-type cyclins and other M phase regulators. We show that an extended prophase I additionally requires the suppression of latent, mitotic cell-cycle controls by the anaphase-promoting complex (APC/C) and its meiosis-specific activator Ama1, which trigger the degradation of M phase regulators and Ndd1, a subunit of a mitotic transcription factor. ama1Δ mutants exit from prophase I prematurely and independently of the RC, which results in recombination defects and chromosome missegregation. Thus, control of prophase I by meiotic mechanisms depends on the suppression of the alternative, mitotic mechanisms by a meiosis-specific form of the APC/C.
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U2 - 10.1016/j.cell.2012.08.044
DO - 10.1016/j.cell.2012.08.044
M3 - Article
C2 - 23101628
AN - SCOPUS:84868033744
SN - 0092-8674
VL - 151
SP - 603
EP - 618
JO - Cell
JF - Cell
IS - 3
ER -