Mechanism of PNA transport to the nuclear compartment

Lenka Stankova, Amy J. Ziemba, Zhanna V. Zhilina, Scot W. Ebbinghaus

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We evaluated the nuclear uptake of fluorescently labeled peptide nucleic acids and measured the binding of unlabeled peptide nucleic acids (PNAs) to the endogenous HER-2/neu promoter in digitonin-permeabilized SK-BR-3 cells. Fluorescently labeled PNAs readily enter the nucleus of digitonin-permeabilized cells, and binding to the chromosomal target sequence was detected with a bis-PNA. Nuclear uptake and target sequence binding were inhibited by N-ethylmaleimide (NEM) and GTPγS. We conclude that PNAs are transported into the nucleus through an energy-dependent process involving the nuclear pore complex.

Original languageEnglish (US)
Title of host publicationOligonucleotide Therapeutics
Subtitle of host publicationFirst Annual Meeting of the Oligonucleotide Therapeutics Society
PublisherBlackwell Publishing Inc.
Number of pages4
ISBN (Print)1573315877, 9781573315876
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632


  • Digitonin
  • GTPγS
  • Karyopherins
  • N-ethylmaleimide
  • NEM
  • Nuclear pore complex
  • Oligonucleotide
  • Peptide nucleic acid
  • Permeabilized cells
  • SK-BR-3 cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science


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