TY - JOUR
T1 - Mechanism of action of atpenin b on raji cells
AU - Oshino, K.
AU - Kumagai, H.
AU - Tomoda, H.
AU - Omura, S.
PY - 1990
Y1 - 1990
N2 - Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13∼0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5′-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting the inhibition of ATP-generating system by atpenin B.
AB - Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 μM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 μM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13∼0.24 μM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5′-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 μM, suggesting the inhibition of ATP-generating system by atpenin B.
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U2 - 10.7164/antibiotics.43.1064
DO - 10.7164/antibiotics.43.1064
M3 - Article
C2 - 2211368
AN - SCOPUS:0025064446
SN - 0021-8820
VL - 43
SP - 1064
EP - 1068
JO - The Journal of Antibiotics
JF - The Journal of Antibiotics
IS - 9
ER -