Mechanical loading regulates NOS expression and activity in developing and adult skeletal muscle

James G. Tidball, Eliane Lavergne, Kim S. Lau, Melissa J. Spencer, James T. Stull, Michelle Wehling

Research output: Contribution to journalArticlepeer-review

142 Scopus citations


The hypothesis that changes in muscle activation and loading regulate the expression and activity of neuronal nitric oxide (NO) synthase (nNOS) was tested using in vitro and in vive approaches. Removal of weight bearing from rat hindlimb muscles for 10 days resulted in a significant decrease in nNOS protein and mRNA concentration in soleus muscles, which returned to control concentrations after return to weight bearing. Similarly, the concentration of nNOS in cultured myotubes increased by application of cyclic loading for 2 days. NO release from excised soleus muscles was increased significantly by a single passive stretch of 20% or by submaximal activation at 2 Hz, although the increases were not additive when both stimuli were applied simultaneously. Increased NO release resulting from passive stretch or activation was dependent on the presence of extracellular calcium. Cyclic loading of cultured myotubes also resulted in a significant increase in NO release. Together, these findings show that activity of muscle influences NO production in the short term, by regulating NOS activity, and in the long term, by regulating nNOS expression.

Original languageEnglish (US)
Pages (from-to)C260-C266
JournalAmerican Journal of Physiology - Cell Physiology
Issue number1 44-1
StatePublished - Jul 1998


  • Calcium
  • Contraction
  • Muscle mechanics
  • Rat

ASJC Scopus subject areas

  • Physiology
  • Cell Biology


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