Abstract
Accurate measurements of rotor temperature are critical for the interpretation of hydrodynamic parameters in analytical ultracentrifugation. We have recently developed methods for a more accurate determination of the temperature of a spinning rotor using iButton temperature loggers. Here we report that the temperature measured with the iButton on the counterbalance of a resting rotor, following thermal equilibration under high vacuum, closely corresponded to the temperature of the spinning rotor with a precision better than 0.2 °C. This strategy offers an inexpensive and straightforward approach to monitor the accuracy of the temperature calibration and determine corrective temperature offsets.
Original language | English (US) |
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Pages (from-to) | 37-39 |
Number of pages | 3 |
Journal | Analytical biochemistry |
Volume | 458 |
DOIs | |
State | Published - Aug 1 2014 |
Keywords
- Hydrodynamics
- Sedimentation velocity
- Temperature calibration
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology