Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

Jeffrey B. Guild; Chris Xu; Watt W. Webb

doi:10.1364/AO.36.000397

Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

Jeffrey B. Guild, Chris Xu, Watt W. Webb

Radiology

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs₂). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.

Original language	English (US)
Pages (from-to)	397-401
Number of pages	5
Journal	Applied Optics
Volume	36
Issue number	1
DOIs	https://doi.org/10.1364/AO.36.000397
State	Published - Jan 1 1997

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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abstract = "We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.",

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N2 - We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.

AB - We determined the group-delay dispersion (GDD) of five microscope objectives by measuring the secondorder autocorrelation at the focal points of the objectives with two-photon excited fluorescence as the power square sensor. We found that typical microscope lens systems introduce significant GDD (2000- 6500 fs2). The third-order dispersion determined for these objectives limits the minimum obtainable pulse width at the focal point of an objective to 20-30 fs if not compensated. No significant chromatic aberration or higher-order dispersion effects were found for any of the optical components measured within the wavelength range of 700-780 nm and for pulse widths greater than 50-60 fs.

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Measurement of group delay dispersion of high numerical aperture objective lenses using two-photon excited fluorescence

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