Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla

Changho Choi; Nicholas J. Coupland; Paramjit P. Bhardwaj; Nikolai Malykhin; Dan Gheorghiu; Peter S. Allen

doi:10.1002/mrm.20875

Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla

Changho Choi, Nicholas J. Coupland, Paramjit P. Bhardwaj, Nikolai Malykhin, Dan Gheorghiu, Peter S. Allen

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

A new single-voxel proton NMR spectrally-selective refocusing method for measuring glutamate (Glu) and glutamine (Gln) in the human brain in vivo at 3T is reported. Triple-resonance selective 180° RF pulses with a bandwidth of 12 Hz were implemented within point-resolved spectroscopy (PRESS) for selective detection of Glu or Gln, and simultaneous acquisition of creatine singlets for use as a reference in phase correction. The carriers of the spectrally-selective 180° pulses and the echo times (TEs) were optimized with both numerical and experimental analyses of the filtering performance, which enabled measurements of the target metabolites with negligible contamination from N-acetylaspartate and glutathione. The concentrations of Glu and Gln in the prefrontal cortex were estimated to be 9.7 ± 0.5 and 3.0 ± 0.7 mM (mean ± SD, N = 7), with reference to Cr at 8 mM.

Original language	English (US)
Pages (from-to)	997-1005
Number of pages	9
Journal	Magnetic resonance in medicine
Volume	55
Issue number	5
DOIs	https://doi.org/10.1002/mrm.20875
State	Published - May 2006

Keywords

3T
Glutamate
Glutamine
Human brain
Prefrontal cortex
RF pulse
Spectrally-selective refocusing
Triple-resonance selective 180°

ASJC Scopus subject areas

Radiology Nuclear Medicine and imaging

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10.1002/mrm.20875

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title = "Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla",

abstract = "A new single-voxel proton NMR spectrally-selective refocusing method for measuring glutamate (Glu) and glutamine (Gln) in the human brain in vivo at 3T is reported. Triple-resonance selective 180° RF pulses with a bandwidth of 12 Hz were implemented within point-resolved spectroscopy (PRESS) for selective detection of Glu or Gln, and simultaneous acquisition of creatine singlets for use as a reference in phase correction. The carriers of the spectrally-selective 180° pulses and the echo times (TEs) were optimized with both numerical and experimental analyses of the filtering performance, which enabled measurements of the target metabolites with negligible contamination from N-acetylaspartate and glutathione. The concentrations of Glu and Gln in the prefrontal cortex were estimated to be 9.7 ± 0.5 and 3.0 ± 0.7 mM (mean ± SD, N = 7), with reference to Cr at 8 mM.",

keywords = "3T, Glutamate, Glutamine, Human brain, Prefrontal cortex, RF pulse, Spectrally-selective refocusing, Triple-resonance selective 180°",

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AU - Coupland, Nicholas J.

AU - Bhardwaj, Paramjit P.

AU - Malykhin, Nikolai

AU - Gheorghiu, Dan

AU - Allen, Peter S.

PY - 2006/5

Y1 - 2006/5

N2 - A new single-voxel proton NMR spectrally-selective refocusing method for measuring glutamate (Glu) and glutamine (Gln) in the human brain in vivo at 3T is reported. Triple-resonance selective 180° RF pulses with a bandwidth of 12 Hz were implemented within point-resolved spectroscopy (PRESS) for selective detection of Glu or Gln, and simultaneous acquisition of creatine singlets for use as a reference in phase correction. The carriers of the spectrally-selective 180° pulses and the echo times (TEs) were optimized with both numerical and experimental analyses of the filtering performance, which enabled measurements of the target metabolites with negligible contamination from N-acetylaspartate and glutathione. The concentrations of Glu and Gln in the prefrontal cortex were estimated to be 9.7 ± 0.5 and 3.0 ± 0.7 mM (mean ± SD, N = 7), with reference to Cr at 8 mM.

AB - A new single-voxel proton NMR spectrally-selective refocusing method for measuring glutamate (Glu) and glutamine (Gln) in the human brain in vivo at 3T is reported. Triple-resonance selective 180° RF pulses with a bandwidth of 12 Hz were implemented within point-resolved spectroscopy (PRESS) for selective detection of Glu or Gln, and simultaneous acquisition of creatine singlets for use as a reference in phase correction. The carriers of the spectrally-selective 180° pulses and the echo times (TEs) were optimized with both numerical and experimental analyses of the filtering performance, which enabled measurements of the target metabolites with negligible contamination from N-acetylaspartate and glutathione. The concentrations of Glu and Gln in the prefrontal cortex were estimated to be 9.7 ± 0.5 and 3.0 ± 0.7 mM (mean ± SD, N = 7), with reference to Cr at 8 mM.

KW - 3T

KW - Glutamate

KW - Glutamine

KW - Human brain

KW - Prefrontal cortex

KW - RF pulse

KW - Spectrally-selective refocusing

KW - Triple-resonance selective 180°

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Measurement of brain glutamate and glutamine by spectrally-selective refocusing at 3-tesla

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Keywords

ASJC Scopus subject areas

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