@inbook{95914d2d9b5b433fb2b5c0ea0dbaa4f5,
title = "Manipulation of renal gene expression using oligonucleotides",
abstract = "Oligonucleotides are small molecules 8–50 nucleotides in length that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA. The use of oligonucleotides to manipulate gene expression in the kidney could be a valuable tool to further understand kidney pathophysiology and can serve as an important complement to genetic studies. This chapter serves as a primer on the use of oligonucleotides in the kidney. We provide an overview of the various ways that oligonucleotides can manipulate gene expression. In addition, we describe the advancements in the development of oligonucleotides for laboratory and clinical use. Finally, instruction is provided on the design and implementation of oligonucleotides for in vitro and in vivo laboratory studies.",
keywords = "Antisense oligonucleotides, Gene expression, MicroRNA, Oligonucleotides, RNA, Therapeutics",
author = "Ronak Lakhia and Abheepsa Mishra and Vishal Patel",
note = "Funding Information: The work from the author's laboratory is supported by National Institute of Health (R01DK102572) and the Department of Defense (D01 W81XWH1810673) to V.P. R.L. is supported by National Institute of Health (K08DK117049). Vishal Patel has applied for a patent related to the treatment of polycystic kidney disease using miR-17 inhibitors. The Patel lab has a sponsored research agreement with Regulus Therapeutics. The remaining authors declare no conflict of interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mcb.2019.05.006",
language = "English (US)",
isbn = "9780128203354",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "109--120",
editor = "Thomas Weimbs",
booktitle = "Methods in Kidney Cell Biology - Part B",
}