Manduca sexta gloverin binds microbial components and is active against bacteria and fungi

Xiao Xia Xu, Xue Zhong, Hui Yu Yi, Xiao Qiang Yu

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Hyalophora gloveri gloverin is a glycine-rich and heat stable antimicrobial protein with activity mainly against Escherichia coli. However, Spodoptera exigua gloverin is active against a Gram-positive bacterium but inactive against E. coli. In this study, we investigated expression profile, binding ability and antimicrobial activity of Manduca sexta gloverin (MsGlv). Msglv transcript was detected in several tissues of naïve larvae with higher levels in the midgut and testis. Expression of Msglv mRNA in larvae was up-regulated by active Spätzle-C108 and peptidoglycans (PGs) of E. coli and Staphylococcus aureus, and the activation was blocked by pre-injection of antibody to M. sexta Toll, suggesting that Msglv expression is regulated by the Toll-Spätzle pathway. Recombinant MsGlv bound to the O-specific antigen and outer core carbohydrate of lipopolysaccharide (LPS), Gram-positive lipoteichoic acid (LTA) and PG, and laminarin, but not to E. coli PG or mannan. MsGlv was active against Bacillus cereus, Saccharomyces cerevisiae and Cryptococcus neoformans, but was almost inactive against E. coli and S. aureus. Our results suggest that gloverins are active against some bacteria and fungi.

Original languageEnglish (US)
Pages (from-to)275-284
Number of pages10
JournalDevelopmental and Comparative Immunology
Issue number2
StatePublished - Oct 2012
Externally publishedYes


  • Antimicrobial activity
  • Gloverin
  • Lipopolysaccharide
  • Lipoteichoic acid
  • Peptidoglycan
  • Toll-Spätzle pathway

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology


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