Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways

Yi Wei Jiang; Philippe Veschambre; Hediye Erdjument-Bromage; Paul Tempst; Joan W. Conaway; Ronald C. Conaway; Roger D. Kornberg

doi:10.1073/pnas.95.15.8538

Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways

Yi Wei Jiang, Philippe Veschambre, Hediye Erdjument-Bromage, Paul Tempst, Joan W. Conaway, Ronald C. Conaway, Roger D. Kornberg

Research output: Contribution to journal › Article › peer-review

279 Scopus citations

Abstract

A multiprotein complex isolated from murine cells is identified as a counterpart of the yeast Mediator of transcriptional regulation on the basis of the following: homologs of two subunits of yeast Mediator, Srb7 and Med7, copurify with the complex; peptide sequencing reveals, in addition, homologs of the yeast Mediator subunits Rgr1 and Med6; as with yeast Mediator, the mouse complex binds to the RNA polymerase II C-terminal domain (CTD) and stimulates phosphorylation of the CTD by TFIIH. Peptide sequencing also identifies a component of mouse Mediator as a relative of Ring-3 protein, a mitogen-activated nuclear protein kinase, raising the possibility of Mediator as an end point of signal transduction pathways.

Original language	English (US)
Pages (from-to)	8538-8543
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	95
Issue number	15
DOIs	https://doi.org/10.1073/pnas.95.15.8538
State	Published - Jul 21 1998
Externally published	Yes

Keywords

C-terminal domain
MED genes
Ring-3
SRB genes
TFIIH kinase

ASJC Scopus subject areas

General

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10.1073/pnas.95.15.8538

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Jiang, Y. W., Veschambre, P., Erdjument-Bromage, H., Tempst, P., Conaway, J. W., Conaway, R. C., & Kornberg, R. D. (1998). Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. Proceedings of the National Academy of Sciences of the United States of America, 95(15), 8538-8543. https://doi.org/10.1073/pnas.95.15.8538

Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. / Jiang, Yi Wei; Veschambre, Philippe; Erdjument-Bromage, Hediye et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 15, 21.07.1998, p. 8538-8543.

Research output: Contribution to journal › Article › peer-review

Jiang, YW, Veschambre, P, Erdjument-Bromage, H, Tempst, P, Conaway, JW, Conaway, RC & Kornberg, RD 1998, 'Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways', Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 15, pp. 8538-8543. https://doi.org/10.1073/pnas.95.15.8538

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AU - Conaway, Ronald C.

AU - Kornberg, Roger D.

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Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways

Abstract

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