@article{03705941a2d9429babea550e974f3d78,
title = "Male germ cells support long-term propagation of Zika virus",
abstract = "Evidence of male-to-female sexual transmission of Zika virus (ZIKV) and viral RNA in semen and sperm months after infection supports a potential role for testicular cells in ZIKV propagation. Here, we demonstrate that germ cells (GCs) are most susceptible to ZIKV. We found that only GCs infected by ZIKV, but not those infected by dengue virus and yellow fever virus, produce high levels of infectious virus. This observation coincides with decreased expression of interferon-stimulated gene Ifi44l in ZIKV-infected GCs, and overexpression of Ifi44l results in reduced ZIKV production. Using primary human testicular tissue, we demonstrate that human GCs are also permissive for ZIKV infection and production. Finally, we identified berberine chloride as a potent inhibitor of ZIKV infection in both murine and human testes. Together, these studies identify a potential cellular source for propagation of ZIKV in testes and a candidate drug for preventing sexual transmission of ZIKV.",
author = "Robinson, {Christopher L.} and Chong, {Angie C.N.} and Ashbrook, {Alison W.} and Ginnie Jeng and Julia Jin and Haiqi Chen and Tang, {Elizabeth I.} and Martin, {Laura A.} and Kim, {Rosa S.} and Kenyon, {Reyn M.} and Eileen Do and Luna, {Joseph M.} and Mohsan Saeed and Lori Zeltser and Harold Ralph and Dudley, {Vanessa L.} and Marc Goldstein and Rice, {Charles M.} and Cheng, {C. Yan} and Marco Seandel and Shuibing Chen",
note = "Funding Information: S.C., a New York Stem Cell Foundation-Robertson Investigator, is funded by The New York Stem Cell Foundation (R-103), and a pilot award from the Center for Advanced Digestive Care of New York Presbyterian Hospital. C.L.R. is funded by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number T32GM007739 and the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number DP2DK098093. M.S. is supported by a grant from the Eunice Kennedy Shriver National Institute of Child Health and Human Development of National Institutes of Health (1DP2HD080352-01) and a grant from the New York State Stem Cell Science Board (C029156). C.Y.C. is supported by a grant from the Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health (R01 HD056034). L.A.M. is a New York Stem Cell Foundation-Druckenmiller Fellow. Generous donations to the Rockefeller University support ZIKV work of J.M.L., A.W.A., and C.M.R. We would like to thank Peter Marzuk, Eva Robinson, Jordan Robinson, and Jason Hoo-Fatt for assistance and support. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41467-018-04444-w",
language = "English (US)",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}