@article{4bad574e91a144fa9b4b448fb7b06c4e,
title = "MAGE-RING protein complexes comprise a family of E3 ubiquitin ligases",
abstract = "The melanoma antigen (MAGE) family consists of more than 60 genes, many of which are cancer-testis antigens that are highly expressed in cancer and play a critical role in tumorigenesis. However, the biochemical and cellular functions of this enigmatic family of proteins have remained elusive. Here, we identify really interesting new gene (RING) domain proteins as binding partners for MAGE family proteins. Multiple MAGE family proteins bind E3 RING ubiquitin ligases with specificity. The crystal structure of one of these MAGE-RING complexes, MAGE-G1-NSE1, reveals structural insights into MAGE family proteins and their interaction with E3 RING ubiquitin ligases. Biochemical and cellular assays demonstrate that MAGE proteins enhance the ubiquitin ligase activity of RING domain proteins. For example, MAGE-C2-TRIM28 is shown to target p53 for degradation in a proteasome-dependent manner, consistent with its tumorigenic functions. These findings define a biochemical and cellular function for the MAGE protein family.",
author = "Doyle, {Jennifer M.} and Jinlan Gao and Jiawei Wang and Maojun Yang and Potts, {Patrick Ryan}",
note = "Funding Information: We would like to thank Hongtao Yu (HHMI and UT Southwestern Medical Center) for support during the early stages of this work and Drs. Lloyd Old (Ludwig Institute for Cancer Research) and Lopra Mishra (Georgetown University) for generously providing the MAGE-C2 and Praja-1 antibodies, respectively. We would also like to thank Jianhua He (Shanghai Sychrotron Radiation Facility BL17U), S. Baba (Spring-8 beamline BL38B1), Yuhui Dong (Beijing Synchrotron Radiation Facility), Na Wang (Tsinghua University), and Fangfang Gao (Tsinghua University) for technical assistance. Finally, we would like to thank Steven McKnight, John Minna, Matthew Porteus, Malia Potts, Michael Rosen, Yigong Shi, and Michael White for helpful discussion and critical reading of the manuscript. This work was supported by Tsinghua University Startup Funds (M.Y.), National Natural Science Foundation of China, National Laboratory Special Fund 2060204 (M.Y.), and the Sara and Frank McKnight Fellowship (P.R.P.). ",
year = "2010",
month = sep,
doi = "10.1016/j.molcel.2010.08.029",
language = "English (US)",
volume = "39",
pages = "963--974",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}