@article{d1c5422391e34906a745bf46216983e6,
title = "Macrophages regulate gastrointestinal motility through complement component 1q",
abstract = "Peristaltic movement of the intestine propels food down the length of the gastrointestinal tract to promote nutrient absorption. Interactions between intestinal macrophages and the enteric nervous system regulate gastrointestinal motility, yet we have an incomplete understanding of the molecular mediator{\textquoteright}s of this crosstalk. Here we identify complement component Iq (Clq) as a macrophage product that regulates gut motility. Macrophages were the predominant source of Clq in the mouse intestine and most extraintestinal tissues. Although Clq mediates complement-mediated killing of bacteria in the bloodstream, we found that Clq was not essential for immune defense of the intestine. Instead. Clq- expressing macrophages were located in the intestinal submucosal and myenteric plexuses where they closely associated with enteric neurons and expressed surface markers characteristic of nerve-adjacent macrophages in other tissues. Mice with a macrophage-specific deletion of Clqa showed changes in enteric neuronal gene expression, increased neurogenic activity of peristalsis, and accelerated intestinal transit.",
author = "Mihir Pendse and {De Selle}, Haley and Nguyen Vo and Gabriella Quinn and Chaitanya Dende and Yun Li and Salinas, {Cristine N.} and Tarun Srinivasan and Propheter, {Daniel C.} and Crofts, {Alexander A.} and Eugene Koo and Brian Hassell and Ruhn, {Kelly A.} and Prithvi Raj and Yuuki Obata and Hooper, {Lora V.}",
note = "Funding Information: was supported by NIH T32 AI005284. A.A.C. was supported by NIH T32 AI005284 and NIH F32 Funding Information: the Harold C. Simmons Cancer Center. The QLMC is supported in part by the National Cancer Institute Funding Information: Cancer Center Support Grant P30 CA142543-01 and NIH 1S10OD028630-01. Citrobacter rodentium Funding Information: We thank Shai Bel for assistance with immunofluorescence imaging experiments. the LT Southwestern Genomics Core for assistance with RNA sequencing expeiiments. the UT Southwestern Flow Cytometry Core for assistance with flow cytometry experiments. Bret Evers (UT Southwestern Histo Pathology Core) for pathology scoring, and the Quantitative Light Microscopy Core (QLMC). a Shared Resource of the Harold C. Simmons Cancer Center. The QLMC is supported in part by the National Cancer Institute Cancer Center Support Grant P30 CA142543-01 and NTH 1S100D028630-01. Citrobacter rodentium strain DBS 100 was a gift from Vanessa Sperandio (UT Southwestern). The laboratory of Pieter Vanden Berghe (University of Leuven Belgium) provided the algorithm used to generate spatiotemporal maps of colonic migrating motor complexes. This work was supported by NIH grants R01 DK070855 (L.V.H.), Welch Foundation Grant 1-1874 (L.VJL), the Walter M. and Helen D. Bader Center for Research on Arthritis and Autoimmune Diseases (L.V.H.), and the Howard Hughes Medical Institute (L.VJL). MP. was supported by NIH T32 AI005284. A.A.C. was supported by NIH T32 AI005284 and NIH F32 DK132913. E.K. was supported by NIH F31 DK126391. Y.O. is the Nancy Cain Marcus and Jeffrey A. Marcus Scholar in Medical Research, in Honor of Dr. Bill S. Vowell. Funding Information: colonic migrating motor complexes. This work was supported by NIH grants R01 DK070855 (L.V.H.), Funding Information: DK132913. E.K. was supported by NIH F31 DK126391. Y.O. is the Nancy Cain Marcus and Jeffrey A. Publisher Copyright: {\textcopyright} 2023, eLife Sciences Publications Ltd. All rights reserved.",
year = "2023",
month = apr,
doi = "10.7554/eLife.78558",
language = "English (US)",
volume = "12",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}