TY - JOUR
T1 - LSm14A is a processing body-associated sensor of viral nucleic acids that initiates cellular antiviral response in the early phase of viral infection
AU - Li, Ying
AU - Chen, Rui
AU - Zhou, Qian
AU - Xu, Zhisheng
AU - Li, Chao
AU - Wang, Shuai
AU - Mao, Aiping
AU - Zhang, Xiaodong
AU - He, Weiwu
AU - Shu, Hong Bing
PY - 2012/7/17
Y1 - 2012/7/17
N2 - Recognition of viral nucleic acids by pattern recognition receptors initiates type I IFN induction and innate antiviral immune response. Here we show that LSm14A, a member of the LSm family involved in RNA processing in the processing bodies, binds to synthetic or viral RNA and DNA and mediates IRF3 activation and IFN-β induction. Knockdown of LSm14A inhibits cytosolic RNA- and DNA-trigger type I IFN production and cellular antiviral response. Moreover, LSm14A is essential for early-phase induction of IFN-βafter either RNA or DNA virus infection. We further found that LSm14A-mediated IFN-β induction requires RIG-I-VISA or MITA after RNA or DNA virus infection, respectively, and viral infection causes translocation of LSm14A to peroxisomes, where RIG-I, VISA, and MITA are located. These findings suggest that LSm14A is a sensor for both viral RNA and DNA and plays an important role in initiating IFN-β induction in the early phase of viral infection.
AB - Recognition of viral nucleic acids by pattern recognition receptors initiates type I IFN induction and innate antiviral immune response. Here we show that LSm14A, a member of the LSm family involved in RNA processing in the processing bodies, binds to synthetic or viral RNA and DNA and mediates IRF3 activation and IFN-β induction. Knockdown of LSm14A inhibits cytosolic RNA- and DNA-trigger type I IFN production and cellular antiviral response. Moreover, LSm14A is essential for early-phase induction of IFN-βafter either RNA or DNA virus infection. We further found that LSm14A-mediated IFN-β induction requires RIG-I-VISA or MITA after RNA or DNA virus infection, respectively, and viral infection causes translocation of LSm14A to peroxisomes, where RIG-I, VISA, and MITA are located. These findings suggest that LSm14A is a sensor for both viral RNA and DNA and plays an important role in initiating IFN-β induction in the early phase of viral infection.
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U2 - 10.1073/pnas.1203405109
DO - 10.1073/pnas.1203405109
M3 - Article
C2 - 22745163
AN - SCOPUS:84863962538
SN - 0027-8424
VL - 109
SP - 11770
EP - 11775
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 29
ER -