LOVTRAP: An optogenetic system for photoinduced protein dissociation

Hui Wang; Marco Vilela; Andreas Winkler; Miroslaw Tarnawski; Ilme Schlichting; Hayretin Yumerefendi; Brian Kuhlman; Rihe Liu; Gaudenz Danuser; Klaus M. Hahn

doi:10.1038/nmeth.3926

LOVTRAP: An optogenetic system for photoinduced protein dissociation

Hui Wang, Marco Vilela, Andreas Winkler, Miroslaw Tarnawski, Ilme Schlichting, Hayretin Yumerefendi, Brian Kuhlman, Rihe Liu, Gaudenz Danuser, Klaus M. Hahn

Research output: Contribution to journal › Article › peer-review

213 Scopus citations

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

Original language	English (US)
Pages (from-to)	755-758
Number of pages	4
Journal	Nature methods
Volume	13
Issue number	9
DOIs	https://doi.org/10.1038/nmeth.3926
State	Published - Aug 30 2016

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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abstract = "LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (K d). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.",

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AU - Winkler, Andreas

AU - Tarnawski, Miroslaw

AU - Schlichting, Ilme

AU - Yumerefendi, Hayretin

AU - Kuhlman, Brian

AU - Liu, Rihe

AU - Danuser, Gaudenz

AU - Hahn, Klaus M.

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LOVTRAP: An optogenetic system for photoinduced protein dissociation

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