Abstract
Phosphorylation of the regulatory light chain of myosin II by myosin light chain kinase is important for regulating many contractile processes. Smooth muscle myosin light chain kinase has been shown to be associated with both actin and myosin filaments in vitro and in vivo. In this report we define an actin binding region by using molecular deletions to generate recombinant mutant proteins that were analyzed by co-sedimentation with F-actin. An actin binding region restricted to residues 2-42 in the animo terminus of the rabbit smooth muscle myosin light chain kinase was identified.
Original language | English (US) |
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Pages (from-to) | 51-57 |
Number of pages | 7 |
Journal | Molecular and Cellular Biochemistry |
Volume | 173 |
Issue number | 1-2 |
DOIs | |
State | Published - 1997 |
Keywords
- Actin binding
- Contraction
- Myosin light chain kinase
- Protein kinase
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology