Abstract
Objective Pulmonary embolism (PE) can cause intracardiac hemolysis and increased plasma hemoglobin and arginase-1, which can worsen pulmonary vasoconstriction. We test the hypothesis that patients with PE that causes tricuspid regurgitation (TR), indicative of higher pulmonary arterial pressures, have decreased leukocyte expression of hmox-1 compared with patients with PE and no TR and patients without PE. Design Prospective, noninterventional study. Patients Normotensive patients with suspected PE (n = 87) who underwent CT pulmonary angiography and transthoracic Doppler-echocardiography. Measurements Significant TR was defined as a jet velocity > 2.7 m/s. Leukocyte expression of hmox-1, haptoglobin, haptoglobin related gene, the haptoglobin receptor, CD163 and cox-2 genes were assessed by quantitative rtPCR, and the hmox-1 promoter was examined for the - 413 A → T SNP and GT repeat polymorphisms. Results Of the 44 (50%) with PE +, 22 had TR +, and their mean pulmonary vascular occlusion (39 ± 32%) did not differ significantly from patients who were TR - (28 ± 26%, P = 0.15). Patients with PE + and TR + had significantly lower expression of hmox-1 and haptoglobin genes than patients without PE + and no TR. Expression of hmox-1 varied inversely with TR velocity (r2 = 0.45, P < 0.001) for PE + (n = 22) but not patients without PE. Hmox-1 expression did not vary significantly with genotype. Cox-2 did not differ between groups and had no correlation with TR. Conclusions Severity of TR varied inversely with hmox-1 expression, suggesting that hmox-1 expression affects pulmonary vascular reactivity after PE.
Original language | English (US) |
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Pages (from-to) | 769-774 |
Number of pages | 6 |
Journal | Thrombosis research |
Volume | 136 |
Issue number | 4 |
DOIs | |
State | Published - Oct 1 2015 |
Externally published | Yes |
Keywords
- Fibrinolysis
- Haptoglobin
- Heme oxygenase
- Hemolysis
- Pulmonary hypertension
ASJC Scopus subject areas
- Hematology