Laser and tandem scanning confocal microscopic studies of rabbit corneal wound healing

H. Ichijima, J. V. Jester, Walter M Petroll, Harrison D Cavanagh

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The process of corneal endothelial wound healing was studied using laser and tandem scanning confocal microscopy (LSCM and TSCM). Following transcorneal freeze (TCF) injury, rabbit corneas were observed using ex vivo LSCM and in vivo TSCM. LSCM revealed the intracellular actin filament organization which, stained with phalloidin‐FITC, in migrating endothelial cells, transformed fibroblast‐like cells, stroma keratocytes, and epithelial cells during wound healing in corneal tissue. The TSCM provided sequential spatial observation of morphologic changes from endothelium to epithelium of the cornea during in vivo cellular repair of wound healing noninvasively on the same cornea without animal sacrifice. Ex vivo LSCM supported the morphologic analysis of the in vivo TSCM observations.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
Issue number3
StatePublished - 1994


  • actin
  • keratocytes
  • laser scanning confocal microscopy
  • rabbit cornea, endothelial cells
  • tandem scanning confocal microscopy
  • transcorneal freeze injury
  • wound healing

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Instrumentation


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