TY - JOUR
T1 - Large-scale DNA-based typing of HLA-A and HLA-B at low resolution is highly accurate specific and reliable
AU - Hurley, Carolyn Katovich
AU - Maiers, M.
AU - Ng, J.
AU - Wagage, D.
AU - Hegland, J.
AU - Baisch, J.
AU - Endres, R.
AU - Fernandez-Vina, M.
AU - Heine, U.
AU - Hsu, S.
AU - Kamoun, M.
AU - Mitsuishi, Y.
AU - Monos, D.
AU - Noreen, H.
AU - Perlee, L.
AU - Rodriguez-Marino, S.
AU - Smith, A.
AU - Stastny, P.
AU - Trucco, M.
AU - Yang, S. Y.
AU - Yu, N.
AU - Holsten, R.
AU - Hartzman, R. J.
AU - Setterholm, M.
PY - 2000
Y1 - 2000
N2 - DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program® unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.
AB - DNA-based typing of HLA class I alleles of the HLA-A and HLA-B loci using sequence-specific oligonucleotide primers and/or probes has been used for the large-scale typing of individuals for the National Marrow Donor Program® unrelated donor registry. Typing was performed by 16 laboratories at a low level of resolution (e.g. A*01, B*07). The results of blinded quality control analysis for the first 12 months of the project show the typing to be highly accurate, specific and reliable. The total error rate based on 11,545 HLA-A and 11,428 HLA-B assignments was 1.1% for HLA-A and 1.9% for HLA-B. This level of accuracy is particularly remarkable because the quality control samples could not be distinguished from 64,180 donor samples tested at the same time by the laboratories.
KW - Bone marrow registry
KW - HLA-A
KW - HLA-B
KW - Polymerase chain reaction
KW - Sequence-specific oligonucleotide typing
KW - Sequence-specific primer typing
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U2 - 10.1034/j.1399-0039.2000.550409.x
DO - 10.1034/j.1399-0039.2000.550409.x
M3 - Article
C2 - 10852387
AN - SCOPUS:0034012915
SN - 2059-2302
VL - 55
SP - 352
EP - 358
JO - HLA
JF - HLA
IS - 4
ER -