TY - JOUR
T1 - L-WNK1 is required for BK channel activation in intercalated cells
AU - Ray, Evan C.
AU - Carrisoza-Gaytan, Rolando
AU - Al-Bataineh, Mohammad
AU - Marciszyn, Allison L.
AU - Nkashama, Lubika J.
AU - Chen, Jingxin
AU - Winfrey, Aaliyah
AU - Griffiths, Shawn
AU - Lam, Tracey R.
AU - Flores, Daniel
AU - Wu, Peng
AU - Wang, Wen Hui
AU - Huang, Chou Long
AU - Subramanya, Arohan R.
AU - Kleyman, Thomas R.
AU - Satlin, Lisa M.
N1 - Publisher Copyright:
© 2021 the American Physiological Society.
PY - 2021/8
Y1 - 2021/8
N2 - Large-conductance Kþ (BK) channels expressed in intercalated cells (ICs) in the aldosterone-sensitive distal nephron (ASDN) mediate flow-induced Kþ secretion. In the ASDN of mice and rabbits, IC BK channel expression and activity increase with a high-Kþ diet. In cell culture, the long isoform of with-no-lysine kinase 1 (L-WNK1) increases BK channel expression and activity. Apical L-WNK1 expression is selectively enhanced in ICs in the ASDN of rabbits on a high-Kþ diet, suggesting that L-WNK1 contributes to BK channel regulation by dietary Kþ. We examined the role of IC L-WNK1 expression in enhancing BK channel activity in response to a high-Kþ diet. Mice with IC-selective deletion of L-WNK1 (IC-L-WNK1-KO) and littermate control mice were placed on a high-Kþ (5% Kþ, as KCl) diet for 10 or more days. IC-L-WNK1-KO mice exhibited reduced IC apical þ subapical a-subunit expression and BK channel-dependent whole cell currents compared with controls. Six-hour urinary Kþ excretion in response a saline load was similar in IC-L-WNK1-KO mice and controls. The observations that IC-L-WNK1-KO mice on a high-Kþ diet have higher blood Kþ concentration and reduced IC BK channel activity are consistent with impaired urinary Kþ secretion, demonstrating that IC L-WNK1 has a role in the renal adaptation to a high-Kþ diet. NEW & NOTEWORTHY When mice are placed on a high-Kþ diet, genetic disruption of the long form of with no lysine kinase 1 (L-WNK1) in intercalated cells reduced relative apical þ subapical localization of the large-conductance Kþ channel, blunted large-conductance Kþ channel currents in intercalated cells, and increased blood Kþ concentration. These data confirm an in vivo role of L-WNK1 in intercalated cells in adaptation to a high-Kþ diet.
AB - Large-conductance Kþ (BK) channels expressed in intercalated cells (ICs) in the aldosterone-sensitive distal nephron (ASDN) mediate flow-induced Kþ secretion. In the ASDN of mice and rabbits, IC BK channel expression and activity increase with a high-Kþ diet. In cell culture, the long isoform of with-no-lysine kinase 1 (L-WNK1) increases BK channel expression and activity. Apical L-WNK1 expression is selectively enhanced in ICs in the ASDN of rabbits on a high-Kþ diet, suggesting that L-WNK1 contributes to BK channel regulation by dietary Kþ. We examined the role of IC L-WNK1 expression in enhancing BK channel activity in response to a high-Kþ diet. Mice with IC-selective deletion of L-WNK1 (IC-L-WNK1-KO) and littermate control mice were placed on a high-Kþ (5% Kþ, as KCl) diet for 10 or more days. IC-L-WNK1-KO mice exhibited reduced IC apical þ subapical a-subunit expression and BK channel-dependent whole cell currents compared with controls. Six-hour urinary Kþ excretion in response a saline load was similar in IC-L-WNK1-KO mice and controls. The observations that IC-L-WNK1-KO mice on a high-Kþ diet have higher blood Kþ concentration and reduced IC BK channel activity are consistent with impaired urinary Kþ secretion, demonstrating that IC L-WNK1 has a role in the renal adaptation to a high-Kþ diet. NEW & NOTEWORTHY When mice are placed on a high-Kþ diet, genetic disruption of the long form of with no lysine kinase 1 (L-WNK1) in intercalated cells reduced relative apical þ subapical localization of the large-conductance Kþ channel, blunted large-conductance Kþ channel currents in intercalated cells, and increased blood Kþ concentration. These data confirm an in vivo role of L-WNK1 in intercalated cells in adaptation to a high-Kþ diet.
KW - Intercalated cells
KW - Large-conductance potassium channel
KW - Potassium secretion
KW - WNK1 kinase
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U2 - 10.1152/AJPRENAL.00472.2020
DO - 10.1152/AJPRENAL.00472.2020
M3 - Article
C2 - 34229479
AN - SCOPUS:85113618956
SN - 1931-857X
VL - 321
SP - F245-F254
JO - American Journal of Physiology - Renal Physiology
JF - American Journal of Physiology - Renal Physiology
IS - 2
ER -