TY - JOUR
T1 - Isolation of antigen-binding virgin and memory B cells
AU - Myers, Christopher D.
AU - Sanders, Virginia M.
AU - Vitetta, Ellen S.
N1 - Funding Information:
It is estimated that 1/10 6 B cells express immunoglobulin (Ig) receptors specific for any given * This work is supported by NIH Grants AI-11851 and AI-21229. ** Address for correspondence: Dr. E.S. Vitetta, Department of Microbiology,U niversity of Texas Health Science Center at Dallas, 5323 Harry Hines Blvd., Dallas, TX 75235, U.S.A.
PY - 1986/8/21
Y1 - 1986/8/21
N2 - One major obstacle in studying the activation of antigen-specific B cells is the small number of B cells reactive with a particular antigenic epitope. In this report, we describe a method by which large numbers of highly purified antigen-binding cells can be obtained. We have shown that by varying the haptenation level of the erythrocytes used for rosetting, we can purify antigen-binding B cells which have different affinities for the antigenic epitope. Thus, memory cells (which have receptors of higher affinity) can be prepared and these cells are essentially free of contaminating virgin cells. The effects of varying the haptenation levels on the red cells used for purifying the B cells can, in turn, be related to the precursor frequency of secreting cells following their activation with T cells and antigen.
AB - One major obstacle in studying the activation of antigen-specific B cells is the small number of B cells reactive with a particular antigenic epitope. In this report, we describe a method by which large numbers of highly purified antigen-binding cells can be obtained. We have shown that by varying the haptenation level of the erythrocytes used for rosetting, we can purify antigen-binding B cells which have different affinities for the antigenic epitope. Thus, memory cells (which have receptors of higher affinity) can be prepared and these cells are essentially free of contaminating virgin cells. The effects of varying the haptenation levels on the red cells used for purifying the B cells can, in turn, be related to the precursor frequency of secreting cells following their activation with T cells and antigen.
KW - B cells, antigen-specific
KW - Lymphocyte
KW - Memory cell
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U2 - 10.1016/0022-1759(86)90502-8
DO - 10.1016/0022-1759(86)90502-8
M3 - Article
C2 - 3489048
AN - SCOPUS:0022451167
SN - 0022-1759
VL - 92
SP - 45
EP - 57
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -