TY - JOUR
T1 - Isolation and characterization of pmk-(1-3)
T2 - Three p38 homologs in Caenorhabditis elegans
AU - Berman, Kevin
AU - McKay, Jim
AU - Avery, Leon
AU - Cobb, Melanie
N1 - Funding Information:
We thank members of the Cobb and Avery laboratories for many helpful discussions and Dionne Ware for administrative assistance. This work was supported by grants from the National Institutes of Health (GM53032 to M.H.C. and HL46154 to L.A.). K.B. was supported in part by an NIH Medical Scientist Training Program grant and the Perot Family Foundation.
PY - 2001
Y1 - 2001
N2 - p38, a member of the mitogen-activated protein kinase (MAPK) superfamily, is activated in response to a variety of cellular stresses and ligands. Since the genome of the nematode C. elegans has been sequenced, we sought to identify and characterize the nematode homolog of mammalian p38. By sequence analysis and RT-PCR, we isolated cDNAs encoding three kinases, PMK-1, PMK-2, and PMK-3, which we call p38 map kinases due to their high sequence identity with p38. The three genes are contiguous on chromosome IV and comprise an operon. By use of a GFP reporter, we found that the promoter of the pmks is active throughout the intestine. An active form of MAPK/ERK kinase 6 (MEK6) phosphorylated and activated recombinant PMK-1 and PMK-2 in vitro. PMK-1 and PMK-2 phosphorylated activating transcription factor-2 (ATF-2), indicating an activity similar to mammalian p38. When transfected into mammalian cells, these kinases, like p38, are stimulated by osmotic stresses.
AB - p38, a member of the mitogen-activated protein kinase (MAPK) superfamily, is activated in response to a variety of cellular stresses and ligands. Since the genome of the nematode C. elegans has been sequenced, we sought to identify and characterize the nematode homolog of mammalian p38. By sequence analysis and RT-PCR, we isolated cDNAs encoding three kinases, PMK-1, PMK-2, and PMK-3, which we call p38 map kinases due to their high sequence identity with p38. The three genes are contiguous on chromosome IV and comprise an operon. By use of a GFP reporter, we found that the promoter of the pmks is active throughout the intestine. An active form of MAPK/ERK kinase 6 (MEK6) phosphorylated and activated recombinant PMK-1 and PMK-2 in vitro. PMK-1 and PMK-2 phosphorylated activating transcription factor-2 (ATF-2), indicating an activity similar to mammalian p38. When transfected into mammalian cells, these kinases, like p38, are stimulated by osmotic stresses.
KW - MAP kinase
KW - MEK
KW - MEKK
KW - Stress response
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U2 - 10.1006/mcbr.2001.0300
DO - 10.1006/mcbr.2001.0300
M3 - Article
C2 - 11703092
AN - SCOPUS:0035197914
SN - 1522-4724
VL - 4
SP - 337
EP - 344
JO - Molecular Cell Biology Research Communications
JF - Molecular Cell Biology Research Communications
IS - 6
ER -