Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry

Alan J. Schroit; Christian Bloy; Jerome Connor; Jean Pierre Cartron

doi:10.1021/bi00497a003

Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry

Alan J. Schroit, Christian Bloy, Jerome Connor, Jean Pierre Cartron

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Abstract

The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, ¹²⁵I-azido-PS, and with an inhibitor of PS transport, ¹²⁵I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

Original language	English (US)
Pages (from-to)	10303-10306
Number of pages	4
Journal	Biochemistry
Volume	29
Issue number	45
DOIs	https://doi.org/10.1021/bi00497a003
State	Published - Nov 1 1990

ASJC Scopus subject areas

Biochemistry

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title = "Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry",

abstract = "The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.",

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year = "1990",

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T1 - Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry

AU - Schroit, Alan J.

AU - Bloy, Christian

AU - Connor, Jerome

AU - Cartron, Jean Pierre

PY - 1990/11/1

Y1 - 1990/11/1

N2 - The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

AB - The human erythrocyte (RBC) Rh blood group system consists of a complex of distinct integral membrane polypeptides with physical properties common to the aminophospholipid transporter responsible for the transbilayer movement of phosphatidylserine (PS) in RBC. To assess the involvement of Rh polypeptides in PS translocation, the aminophospholipid translocase was labeled with a photoactivatable PS analogue, 125I-azido-PS, and with an inhibitor of PS transport, 125I-labeled 2-(2-pyridyldithio)ethylamine. The ability of monoclonal Rh antibodies to immunoprecipitate the labeled transporter was determined. Immunoprecipitated Rh polypeptides were found to be labeled with the aminophospholipid translocase markers, suggesting that Rh proteins are involved in the transbilayer movement of PS.

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Involvement of Rh Blood Group Polypeptides in the Maintenance of Aminophospholipid Asymmetry

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