Investigation of suspected nosocomial clusters of staphylococcus Haemolyticus infections

Trish M. Perl, Wolfgang A. Krüger, Alison Houston, Linda D. Boyken, Michael A. Pfaller, Loreen A. Herwaldt

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


OBJECTIVE: To determine whether typing methods can discriminate among Staphylococcus haemolyticus isolates. DESIGN: Molecular epidemiological evaluation of S haemolyticus isolates obtained from patients hospitalized on a hematology service and in a surgical intensive-care unit (SICU). SETTING: A large Midwestern teaching hospital. INTERVENTIONS: None. RESULTS: Over 22 days, S haemolyticus was isolated from five patients on the hematology service. Isolates from four patients had the same unusual antibiogram and biotype. Ribotyping, restriction endonuclease digestion of plasmid DNA (REAP), and whole chromosomal DNA analysis by pulsed-field gel electrophoresis (PFGE) confirmed that these isolates were identical and different from the fifth patient's isolate and from 6 control isolates. In a second cluster, 11 S haemolyticus isolates obtained from eight patients in the SICU had similar antibiograms and biotypes. By REAP and ribotype analysis, isolates from four patients were identical. However, PFGE indicated that only two of these patients shared a common strain. CONCLUSIONS: Antibiograms or biotyping may discriminate among isolates of S haemolyticus if the results of these tests are unusual. Many clinical isolates can be differentiated by REAP analysis, ribotyping, or PFGE. However, some isolates are identical by all of these methods, suggesting that they may have been transmilled nosocomially.

Original languageEnglish (US)
Pages (from-to)128-131
Number of pages4
JournalInfection Control and Hospital Epidemiology
Issue number2
StatePublished - 1999

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases


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