TY - JOUR
T1 - Investigation of endosome and lysosome biology by ultra pH-sensitive nanoprobes
AU - Wang, Chensu
AU - Zhao, Tian
AU - Li, Yang
AU - Huang, Gang
AU - White, Michael A.
AU - Gao, Jinming
N1 - Funding Information:
This work is supported by the National Institutes of Health (NIH) grants R01CA192221 (J.G.), R01CA71443 and R01CA176284 (M.A.W.), Cancer Prevention and Research Institute of Texas (CPRIT) grants RP140140 (J.G.), RP121067, RP110710 (M.A.W.), and the Welch Foundation grant I-1414 (M.A.W.). C.W. is a Howard Hughes Medical Institute (HHMI) International Student Research Fellow.
Funding Information:
This work is supported by the National Institutes of Health (NIH) grants R01CA192221 (J.G.), R01CA71443 and R01CA176284 (M.A.W.), Cancer Prevention and Research Institute of Texas (CPRIT) grants RP140140 (J.G.), RP121067 , RP110710 (M.A.W.), and the Welch Foundation grant I-1414 (M.A.W.). C.W. is a Howard Hughes Medical Institute (HHMI) International Student Research Fellow.
Publisher Copyright:
© 2016
PY - 2017/4
Y1 - 2017/4
N2 - Endosomes and lysosomes play a critical role in various aspects of cell physiology such as nutrient sensing, receptor recycling, protein/lipid catabolism, and cell death. In drug delivery, endosomal release of therapeutic payloads from nanocarriers is also important in achieving efficient delivery of drugs to reach their intracellular targets. Recently, we invented a library of ultra pH-sensitive (UPS) nanoprobes with exquisite fluorescence response to subtle pH changes. The UPS nanoprobes also displayed strong pH-specific buffer effect over small molecular bases with broad pH responses (e.g., chloroquine and NH4Cl). Tunable pH transitions from 7.4 to 4.0 of UPS nanoprobes cover the entire physiological pH of endocytic organelles (e.g., early and late endosomes) and lysosomes. These unique physico-chemical properties of UPS nanoprobes allowed a ‘detection and perturbation’ strategy for the investigation of luminal pH in cell signaling and metabolism, which introduces a nanotechnology-enabled paradigm for the biological studies of endosomes and lysosomes.
AB - Endosomes and lysosomes play a critical role in various aspects of cell physiology such as nutrient sensing, receptor recycling, protein/lipid catabolism, and cell death. In drug delivery, endosomal release of therapeutic payloads from nanocarriers is also important in achieving efficient delivery of drugs to reach their intracellular targets. Recently, we invented a library of ultra pH-sensitive (UPS) nanoprobes with exquisite fluorescence response to subtle pH changes. The UPS nanoprobes also displayed strong pH-specific buffer effect over small molecular bases with broad pH responses (e.g., chloroquine and NH4Cl). Tunable pH transitions from 7.4 to 4.0 of UPS nanoprobes cover the entire physiological pH of endocytic organelles (e.g., early and late endosomes) and lysosomes. These unique physico-chemical properties of UPS nanoprobes allowed a ‘detection and perturbation’ strategy for the investigation of luminal pH in cell signaling and metabolism, which introduces a nanotechnology-enabled paradigm for the biological studies of endosomes and lysosomes.
KW - Cell signaling
KW - Endocytic organelles
KW - Metabolomics
KW - Organelle imaging
KW - pH buffering
KW - pH sensitive nanoprobes
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U2 - 10.1016/j.addr.2016.08.014
DO - 10.1016/j.addr.2016.08.014
M3 - Review article
C2 - 27612550
AN - SCOPUS:84994881643
SN - 0169-409X
VL - 113
SP - 87
EP - 96
JO - Advanced Drug Delivery Reviews
JF - Advanced Drug Delivery Reviews
ER -