Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II

Pratibha Bhalla, Dipti Vinayak Vernekar, Benoit Gilquin, Yohann Couté, Purnima Bhargava

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Eukaryotic transcription is a highly regulated fundamental life process. A large number of regulatory proteins and complexes, many of them with sequence-specific DNA-binding activity are known to influence transcription by RNA polymerase (pol)II with a fine precision. In comparison, only a few regulatory proteins are known for pol III, which transcribes genes encoding small, stable, non-translated RNAs. The pol III transcription is precisely regulated under various stress conditions. We used pol III transcription complex (TC)components TFIIIC (Tfc6), pol III (Rpc128)and TFIIIB (Brf1)as baits and mass spectrometry to identify their potential interactors in vivo. A large interactome constituting chromatin modifiers, regulators and factors of transcription by pol I and pol II supports the possibility of a crosstalk between the three transcription machineries. The association of proteins and complexes involved in various basic life processes like ribogenesis, RNA processing, protein folding and degradation, DNA damage response, replication and transcription underscores the possibility of the pol III TC serving as a signaling hub for communication between the transcription and other cellular physiological activities under normal growth conditions. We also found an equally large number of proteins and complexes interacting with the TC under nutrient starvation condition, of which at least 25% were non-identical under the two conditions. The data reveal the possibility of a large number of signaling cues for pol III transcription against adverse conditions, necessary for an efficient co-ordination of various cellular functions.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalGene
Volume702
DOIs
StatePublished - Jun 20 2019
Externally publishedYes

Keywords

  • Chromatin modifiers
  • Interactome
  • RNA polymerase III
  • TFIIIB
  • TFIIIC
  • Transcription
  • Yeast

ASJC Scopus subject areas

  • Genetics

Fingerprint

Dive into the research topics of 'Interactome of the yeast RNA polymerase III transcription machinery constitutes several chromatin modifiers and regulators of the genes transcribed by RNA polymerase II'. Together they form a unique fingerprint.

Cite this