@article{686856f628df41e281353a4ec7caa0e0,
title = "Insulin receptor-related receptor expression in non-A intercalated cells in the kidney",
abstract = "Insulin receptor- related receptor (IRR) is a novel receptor tyrosine kinase in the insulin receptor family. Previous studies have demonstrated that the mammalian organ with the highest level of IRR mRNA is the kidney. By in situ hybridization, kidney expression of IRR transcript is only in the distal nephron and the collecting ducts; however, the specific cellular distribution of IRR is unknown. The purpose of this study was to examine IRR protein expression in the adult mouse kidney using immunohistochemical techniques. IRR was specifically present in a subset of cells in the connecting tubule, the initial collecting tubule, and the cortical collecting duct. IRR protein is detected in cells that express vacuolar H+-ATPase and carbonic anhydrase 2, but not in cells that express band 3 (anion exchanger 1). In the cortical collecting duct, the IRR positive cells are likely B intercalated cells. In the connecting tubule and the initial collecting tubule, the cells are B cells and/or non-A non-B cells. Thus, IRR is a specific marker for non-A intercalated cells in the kidney.",
keywords = "Insulin receptor, Non-A intercalated cells, Receptors, Tyrosine kinase",
author = "Bates, {Carlton M.} and Merenmies, {Jussi M.} and Kelly-Spratt, {Karen S.} and Parada, {Luis F.}",
note = "Funding Information: Physiology and Pathophysiology (2nd ed), edited by Seldin DW, Giebisch G, New York, Raven, 1992, p 708; used with permission). Numbers represent: 1 = cortex; 2 = outer medullary stripe; 3 = inner medullaty stripe; 4 = papilla; 5 = pelvic septum; 6 = pelvic cavity; 7 = hilar tunnel; the box in the lower right corner represents the area shown in B. (B) Low power fluorescent micrograph illustrating IRR staining pattern (magnification X 100). (C) Higher power fluorescent micrograph of IRR staining pattern (magnification X400) (D) Higher power light micrograph demonstrating IRR expression with DAB/NiCl (magnification x400). Symbols are: concave arrowheads, glomeruli; arrowheads, IRR-positive tubular cells; arrows, IRR-negative cells. Reproduction of this figure in color was supported by a grant from the Sandoz Transplant Division of Novartis Pharmaceuticals, East Hanover, NJ, USA. Funding Information: This study was supported by grants from the National institutes of Funding Information: Health, T32 DK07659 and 1-RO1-NS33199. The authors are grateful to Sandoz Transplant Division, Novartis Pharmacenticals for a grant to publish Fig. 2 in color, and to Baxter Healthcare Corporation for a grant to publish Fig. 3 in color. The authors wish to thank Dr. Michel Baum for reviewing the manuscript and offering suggestions, and Dr. Raymond Quigley and Dr. Sadeq Kharzai for helpful discussions. The authors thank Dr. Dennis Stone for the antibody against the H-ATPase, Dr. Seth Alper and Dr. Alan Stuart-Tilley for antibody against AE2, Dr. Evan Stubbs and Dr. George Siegel for antibody against the sodium-potassium ATPase, and Dr. Kirsten Madsen and Dr. Paul Linser for the antibody against carbonic anhydrase 2. The authors thank Zhi Wang for his assistance in the generation of the IRR antibodies.",
year = "1997",
doi = "10.1038/ki.1997.381",
language = "English (US)",
volume = "52",
pages = "674--681",
journal = "Kidney international",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "3",
}