Inhibition of testosterone secretion by S-petasin in rat testicular interstitial cells

Ho Lin, Chau Heng Chien, Yun Lian Lin, Chieh Fu Chen, Paulus S. Wang

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


S-petasin, a kind of sesquiterpene ester, is the anti-inflammatory and analgesic component of the butterbur (Petasites hybridus). The clinical benefit of S-petasin is the spasmolytic activity, but its side effects on the reproductive endocrinology are not clear yet. The present study was to explore the effects of S-petasin on the secretion of testosterone in vivo and in vitro. We found that single intravenous injection of S-petasin (1 μg/kg) decreased basal plasma testosterone concentration in adult male rats. The enzymatically dispersed rat testicular interstitial cells were incubated with S-petasin (0∼4.3×10 -5 M) in the presence or absence of human chorionic gonadotropin (hCG, 0.05 IU/ml), forskolin (adenylyl cyclase activator, 10 -5 M), and androstenedione (testosterone biosynthesis precursor, 10 -9 M) at 34°C for 1 h. The concentrations of testosterone in the incubation medium were measured by radioimmunoassay. S-petasin at 4.3×10 -7 M was effective to reduce the basal and hCG-stimulated release of testosterone in rat testicular interstitial cells. The stimulatory effects of testosterone secretion induced by forskolin and androstenedione were significantly reduced by S-petasin at 4.3×10 -5 M and 4.3×10 -6 M, respectively. These results suggest that S-petasin inhibits the production of testosterone in rat testicular interstitial cells in part through diminishing the activities of adenylyl cyclase and 17-ketosteroid reductase.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalChinese Journal of Physiology
Issue number3
StatePublished - Sep 30 2000


  • Rat
  • S-petasin
  • Testicular interstitial cells
  • Testosterone

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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