TY - JOUR
T1 - Inhibition of myogenic differentiation by fibroblast growth factor or type β transforming growth factor does not require persistent c-myc expression
AU - Spizz, Gwendolyn
AU - Hu, Jing Shan
AU - Olson, Eric N.
N1 - Funding Information:
This investigation was supported by National Institutes of Health Comprehensive Core Support Grant CA 16671; an American Heart Association, Texas Affiliate, Grant G-724, and a Basil O’Connor Starter Research Grant from the March of Dimes. We thank Ellen Madson, Gertrude Willis, and Harriette Young for their expert preparation of this manuscript. We also thank Arnold Strauss for R21.
PY - 1987/10
Y1 - 1987/10
N2 - Skeletal muscle differentiation is accompanied by accumulation of the mRNA encoding the muscle isoenzyme of creatine kinase (MCK) and can be suppressed by serum components, fibroblast growth factor (FGF), or type β transforming growth factor (TGFβ). Using the nonfusing myogenic cell line, BC3H1, the potential involvement of c-myc in growth factor-dependent inhibition of myogenesis was examined. Withdrawal of undifferentiated myoblasts from the cell cycle in medium with 0.5% serum was associated with a precipitous decline in expression of c-myc mRNA followed by induction of MCK mRNA. In 0.5% serum containing TGFβ, c-myc mRNA declined to a level identical to that in differentiated cells; however, MCK mRNA was not expressed. Exposure of quiescent differentiated cells to FGF or TGFβ caused disappearance of muscle-specific gene products and was accompanied by only transient low level induction of c-myc mRNA. These data indicate that persistent c-myc expression is not required for growth factor-mediated inhibition of myogenic differentiation.
AB - Skeletal muscle differentiation is accompanied by accumulation of the mRNA encoding the muscle isoenzyme of creatine kinase (MCK) and can be suppressed by serum components, fibroblast growth factor (FGF), or type β transforming growth factor (TGFβ). Using the nonfusing myogenic cell line, BC3H1, the potential involvement of c-myc in growth factor-dependent inhibition of myogenesis was examined. Withdrawal of undifferentiated myoblasts from the cell cycle in medium with 0.5% serum was associated with a precipitous decline in expression of c-myc mRNA followed by induction of MCK mRNA. In 0.5% serum containing TGFβ, c-myc mRNA declined to a level identical to that in differentiated cells; however, MCK mRNA was not expressed. Exposure of quiescent differentiated cells to FGF or TGFβ caused disappearance of muscle-specific gene products and was accompanied by only transient low level induction of c-myc mRNA. These data indicate that persistent c-myc expression is not required for growth factor-mediated inhibition of myogenic differentiation.
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U2 - 10.1016/0012-1606(87)90408-8
DO - 10.1016/0012-1606(87)90408-8
M3 - Article
C2 - 3477514
AN - SCOPUS:0023500759
SN - 0012-1606
VL - 123
SP - 500
EP - 507
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -