Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription

Bing Li, Madelaine Gogol, Mike Carey, Samantha G. Pattenden, Chris Seidel, Jerry L. Workman

Research output: Contribution to journalArticlepeer-review

150 Scopus citations


The presence of Set2-mediated methylation of H3K36 (K36me) correlates with transcription frequency throughout the yeast genome. K36me targets the Rpd3S complex to deacetylate transcribed regions and suppress cryptic transcription initiation at certain genes. Here, using a genome-wide approach, we report that the Set2-Rpd3S pathway is generally required for controlling acetylation at coding regions. When using acetylation as a functional readout for this pathway, we discovered that longer genes and, surprisingly, genes transcribed at lower frequency exhibit a stronger dependency. Moreover, a systematic screen using high-resolution tiling microarrays allowed us to identify a group of genes that rely on Set2-Rpd3S to suppress spurious transcripts. Interestingly, most of these genes are within the group that depend on the same pathway to maintain a hypoacetylated state at coding regions. These data highlight the importance of using the functional readout of histone codes to define the roles of specific pathways.

Original languageEnglish (US)
Pages (from-to)1422-1430
Number of pages9
JournalGenes and Development
Issue number11
StatePublished - Jun 1 2007


  • Chromatin
  • Cryptic transcripts
  • Deacetylation
  • Histone methylation
  • Rpd3
  • Set2

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


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