Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms

Junyi Lei; Tong Tong Zou; Ying Qiang Shi; Xiaoling Zhou; Kara N. Smolinski; Jing Yin; Rhonda F. Souza; Rebecca Appel; Suna Wang; Karina Cymes; Olivia Chan; John M. Abraham; Noam Harpaz; Stephen J. Meltzer

Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms

Junyi Lei, Tong Tong Zou, Ying Qiang Shi, Xiaoling Zhou, Kara N. Smolinski, Jing Yin, Rhonda F. Souza, Rebecca Appel, Suna Wang, Karina Cymes, Olivia Chan, John M. Abraham, Noam Harpaz, Stephen J. Meltzer

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Homozygously Deleted in Pancreatic Cancer 4 (DPC4), a recently identified candidate tumor suppressor gene, was previously shown to be altered in human pancreatic cancers. We examined DPC4 mutation in 30 examples of three other types of gastrointestinal malignancy: 10 esophageal cancers, 10 gastric cancers and 10 colorectal cancers occurring in the preneoplastic condition, ulcerative colitis. The entire coding region of DPC4 (including all 11 exons) was analysed by either direct sequencing of PCR product or the in vitro synthesized protein assay. No coding region mutations of DPC4 were found in any of the samples examined. Our results suggest that inactivation of DPC4 may not be important in the majority of these types of gastrointestinal cancer.

Original language	English (US)
Pages (from-to)	2459-2462
Number of pages	4
Journal	Oncogene
Volume	13
Issue number	11
State	Published - 1996

Keywords

DNA sequencing
DPC4
Esophageal cancer
Gastric cancer
Mutation
Tumor suppressor gene

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

Cite this

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title = "Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms",

abstract = "Homozygously Deleted in Pancreatic Cancer 4 (DPC4), a recently identified candidate tumor suppressor gene, was previously shown to be altered in human pancreatic cancers. We examined DPC4 mutation in 30 examples of three other types of gastrointestinal malignancy: 10 esophageal cancers, 10 gastric cancers and 10 colorectal cancers occurring in the preneoplastic condition, ulcerative colitis. The entire coding region of DPC4 (including all 11 exons) was analysed by either direct sequencing of PCR product or the in vitro synthesized protein assay. No coding region mutations of DPC4 were found in any of the samples examined. Our results suggest that inactivation of DPC4 may not be important in the majority of these types of gastrointestinal cancer.",

keywords = "DNA sequencing, DPC4, Esophageal cancer, Gastric cancer, Mutation, Tumor suppressor gene",

author = "Junyi Lei and Zou, {Tong Tong} and Shi, {Ying Qiang} and Xiaoling Zhou and Smolinski, {Kara N.} and Jing Yin and Souza, {Rhonda F.} and Rebecca Appel and Suna Wang and Karina Cymes and Olivia Chan and Abraham, {John M.} and Noam Harpaz and Meltzer, {Stephen J.}",

year = "1996",

language = "English (US)",

volume = "13",

pages = "2459--2462",

journal = "Oncogene",

issn = "0950-9232",

publisher = "Nature Publishing Group",

number = "11",

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TY - JOUR

T1 - Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms

AU - Lei, Junyi

AU - Zou, Tong Tong

AU - Shi, Ying Qiang

AU - Zhou, Xiaoling

AU - Smolinski, Kara N.

AU - Yin, Jing

AU - Souza, Rhonda F.

AU - Appel, Rebecca

AU - Wang, Suna

AU - Cymes, Karina

AU - Chan, Olivia

AU - Abraham, John M.

AU - Harpaz, Noam

AU - Meltzer, Stephen J.

PY - 1996

Y1 - 1996

N2 - Homozygously Deleted in Pancreatic Cancer 4 (DPC4), a recently identified candidate tumor suppressor gene, was previously shown to be altered in human pancreatic cancers. We examined DPC4 mutation in 30 examples of three other types of gastrointestinal malignancy: 10 esophageal cancers, 10 gastric cancers and 10 colorectal cancers occurring in the preneoplastic condition, ulcerative colitis. The entire coding region of DPC4 (including all 11 exons) was analysed by either direct sequencing of PCR product or the in vitro synthesized protein assay. No coding region mutations of DPC4 were found in any of the samples examined. Our results suggest that inactivation of DPC4 may not be important in the majority of these types of gastrointestinal cancer.

AB - Homozygously Deleted in Pancreatic Cancer 4 (DPC4), a recently identified candidate tumor suppressor gene, was previously shown to be altered in human pancreatic cancers. We examined DPC4 mutation in 30 examples of three other types of gastrointestinal malignancy: 10 esophageal cancers, 10 gastric cancers and 10 colorectal cancers occurring in the preneoplastic condition, ulcerative colitis. The entire coding region of DPC4 (including all 11 exons) was analysed by either direct sequencing of PCR product or the in vitro synthesized protein assay. No coding region mutations of DPC4 were found in any of the samples examined. Our results suggest that inactivation of DPC4 may not be important in the majority of these types of gastrointestinal cancer.

KW - DNA sequencing

KW - DPC4

KW - Esophageal cancer

KW - Gastric cancer

KW - Mutation

KW - Tumor suppressor gene

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M3 - Article

C2 - 8957088

AN - SCOPUS:8044247811

SN - 0950-9232

VL - 13

SP - 2459

EP - 2462

JO - Oncogene

JF - Oncogene

IS - 11

ER -

Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer and ulcerative colitis-associated neoplasms

Abstract

Keywords

ASJC Scopus subject areas

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