Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

Michael G. Roth; Richard W. Compans; Lorraine Giusti; Alan R. Davis; Debi P. Nayak; Mary Jane Gething; Joe Sambrook

doi:10.1016/0092-8674(83)90425-7

Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

Michael G. Roth, Richard W. Compans, Lorraine Giusti, Alan R. Davis, Debi P. Nayak, Mary Jane Gething, Joe Sambrook

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process.

Original language	English (US)
Pages (from-to)	435-443
Number of pages	9
Journal	Cell
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(83)90425-7
State	Published - Jun 1983

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(83)90425-7

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title = "Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA",

abstract = "Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process.",

author = "Roth, {Michael G.} and Compans, {Richard W.} and Lorraine Giusti and Davis, {Alan R.} and Nayak, {Debi P.} and Gething, {Mary Jane} and Joe Sambrook",

note = "Funding Information: We thank Mr. L. Ft. Melsen for excellent technical assistance, Dr. George Fareed for his advice and valuable suggestions, and Dr. Graham Wilcox for advice on primary cell cultures. This work was supported by grants from the National Science Foundation (PCM 78 23220 and PCM 80 06498) U. S. Public Health Service National Research Service Awards (GM-07104 and Al 07150) and USPHS grants from the National Institute of Allergy and Infectious Diseases (Al 12680 and Al 16348) and the National Cancer Institute (CA 13148).",

year = "1983",

month = jun,

doi = "10.1016/0092-8674(83)90425-7",

language = "English (US)",

volume = "33",

pages = "435--443",

journal = "Cell",

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T1 - Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

AU - Roth, Michael G.

AU - Compans, Richard W.

AU - Giusti, Lorraine

AU - Davis, Alan R.

AU - Nayak, Debi P.

AU - Gething, Mary Jane

AU - Sambrook, Joe

N1 - Funding Information: We thank Mr. L. Ft. Melsen for excellent technical assistance, Dr. George Fareed for his advice and valuable suggestions, and Dr. Graham Wilcox for advice on primary cell cultures. This work was supported by grants from the National Science Foundation (PCM 78 23220 and PCM 80 06498) U. S. Public Health Service National Research Service Awards (GM-07104 and Al 07150) and USPHS grants from the National Institute of Allergy and Infectious Diseases (Al 12680 and Al 16348) and the National Cancer Institute (CA 13148).

PY - 1983/6

Y1 - 1983/6

N2 - Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process.

AB - Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process.

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Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

Abstract

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