@article{95f6d50672e043969e94bf36ebf8f566,
title = "Increasing tumor antigen expression overcomes {"}ignorance{"} to solid tumors via crosspresentation by bone marrow-derived stromal cells",
abstract = "To explain why solid cancers grow or are rejected, we examined how the tumor stroma affected the level of antigen expression necessary to induce an immune response. We applied a tamoxifen-regulated Cre-loxP system to induce a model SIYRYYGL antigen recognized by the 2C T cell receptor. Solid tumors expressing the antigen at lower levels grew, whereas solid tumors expressing antigen induced to 26-fold higher levels were rejected. In contrast, mice rejected cell suspensions expressing higher or lower levels of the antigen. The antigen was likely crosspresented because draining lymph node responses required bone marrow-derived cells in the tumor stroma. Thus, tumor antigens expressed at levels sufficient for crosspresentation by bone marrow-derived stromal cells may overcome immunological {"}ignorance{"} to solid tumors.",
author = "Spiotto, {Michael T.} and Ping Yu and Rowley, {Donald A.} and Nishimura, {Michael I.} and Meredith, {Stephen C.} and Gajewski, {Thomas F.} and Fu, {Yang Xin} and Hans Schreiber",
note = "Funding Information: We thank Thierry Boon, Pamela Ohashi, and Hans Hengartner for the cancer cells lines; Philip Ashton-Rickardt for the P14 mice; Matthew Mescher for the OT-1 mice; Michael Reth for the MerCreMer expression vector; Helene Auer for synthesis of the SIYRYYGL and SNFVFAGI peptides; Candace Cham and Karin Schreiber for help with 2C T cell culture techniques; The University of Chicago Immunology Applications Core Facility for technical assistance with flow cytometry; and Gabrielle Beck-Engesser, Terry Wu, and the rest of the Schreiber lab for technical assistance and advice. This work was supported by National Institutes of Health grants RO1-CA22677, RO1-CA37516, and PO1-CA97296, a grant by the Cancer Research Institute, and by the University of Chicago Cancer Center Grant CA-14599. M.T.S. is a recipient of the Growth and Development Training Grant HD 07009.",
year = "2002",
month = dec,
day = "1",
doi = "10.1016/S1074-7613(02)00480-6",
language = "English (US)",
volume = "17",
pages = "737--747",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "6",
}