Increased serum matrix metalloproteinase-9 levels are associated with Anti-JO1 but not Anti-MDA5 in myositis patients

Yanjuan Liu, Hui Luo, Li Wang, Caiyan Li, Liyun Liu, Li Huang, Ke Liu, Meidong Liu, Siming Gao, Yizhi Xiao, Honglin Zhu, Xiaoxia Zuo, Huali Zhang, Quan Zhen Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Matrix metalloproteinases 9 (MMP9) is a member of the zinc-ion–dependent proteinases family and plays a pathogenic role in chronic inflammatory autoimmune diseases. However, its roles in the pathogenesis of myositis have not been elucidated. In this study, we aimed to determine the gene expression and serum level of MMP9 and their relationship with clinical features and serological parameters in myositis. Our results showed that MMP9 mRNA in peripheral blood mononuclear cells (PBMC) was upregulated in myositis patients compared to that in healthy controls. Myositis patients positive for anti-Jo1 antibodies exhibited significantly higher serum MMP9 than anti-MDA5 positive or antibody-negative patients and healthy controls. However, the presence of interstitial lung disease (ILD) did not affect MMP9 levels. We further identified that anti-Jo1-positive myositis patients showed higher numbers of white blood cells (WBC), lymphocytes and neutrophils; increased levels of creatine kinase (CK), lactate dehydrogenase (LDH), and C-reactive protein (CRP); and higher erythrocyte sedimentation rate (ESR) than anti-MDA5 positive patients. In addition, serum MMP-9 levels were positively correlated with WBCs, neutrophils, CK, CRP, ESR, and LDH in myositis patients. In vitro experiments showed that purified serum IgG from Jo-1-positive patients could stimulate PBMCs to release more MMP9 than the IgG from MDA-5-positive sera. These results indicated that increased MMP9 in anti-Jo1-positive myositis patients was associated with the extent of muscle involvement, but not pulmonary damage. The distinct pattern of serum MMP9 perhaps clarifies the differences inpathophysiology between anti-Jo1 and anti-MDA5 in patients with myositis.

Original languageEnglish (US)
Pages (from-to)746-755
Number of pages10
JournalAging and Disease
Issue number4
StatePublished - 2019
Externally publishedYes


  • Anti-MDA5 antibody; Anti-Jo1 antibody
  • MMP9
  • Myositis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Geriatrics and Gerontology
  • Clinical Neurology
  • Cell Biology


Dive into the research topics of 'Increased serum matrix metalloproteinase-9 levels are associated with Anti-JO1 but not Anti-MDA5 in myositis patients'. Together they form a unique fingerprint.

Cite this