In vivo enzymology: 13C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b

Ralph P. Mason; Jeremy K M Sanders; Alex Cornish

doi:10.1016/0014-5793(87)80745-7

In vivo enzymology: ¹³C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b

Ralph P. Mason, Jeremy K M Sanders, Alex Cornish

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The competitive oxidation of ¹³CH₃OH and ¹³CD₃OH has been observed using in vivo ¹³C NMR spectroscopy. Simultaneous ¹H and ²H decoupling gave isotopically shifted ¹³C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.

Original language	English (US)
Pages (from-to)	4-6
Number of pages	3
Journal	FEBS Letters
Volume	216
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80745-7
State	Published - May 25 1987

Keywords

Enzyme kinetics
Formaldehyde production
Isotope effect
Methanol oxidation
NMR

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(87)80745-7

Cite this

@article{9d3ccb69fada45e689174aad80417b6d,

title = "In vivo enzymology: 13C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b",

abstract = "The competitive oxidation of 13CH3OH and 13CD3OH has been observed using in vivo 13C NMR spectroscopy. Simultaneous 1H and 2H decoupling gave isotopically shifted 13C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.",

keywords = "Enzyme kinetics, Formaldehyde production, Isotope effect, Methanol oxidation, NMR",

author = "Mason, {Ralph P.} and Sanders, {Jeremy K M} and Alex Cornish",

year = "1987",

month = may,

day = "25",

doi = "10.1016/0014-5793(87)80745-7",

language = "English (US)",

volume = "216",

pages = "4--6",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - In vivo enzymology

T2 - 13C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b

AU - Mason, Ralph P.

AU - Sanders, Jeremy K M

AU - Cornish, Alex

PY - 1987/5/25

Y1 - 1987/5/25

N2 - The competitive oxidation of 13CH3OH and 13CD3OH has been observed using in vivo 13C NMR spectroscopy. Simultaneous 1H and 2H decoupling gave isotopically shifted 13C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.

AB - The competitive oxidation of 13CH3OH and 13CD3OH has been observed using in vivo 13C NMR spectroscopy. Simultaneous 1H and 2H decoupling gave isotopically shifted 13C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.

KW - Enzyme kinetics

KW - Formaldehyde production

KW - Isotope effect

KW - Methanol oxidation

KW - NMR

UR - http://www.scopus.com/inward/record.url?scp=45949117165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45949117165&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)80745-7

DO - 10.1016/0014-5793(87)80745-7

M3 - Article

AN - SCOPUS:45949117165

SN - 0014-5793

VL - 216

SP - 4

EP - 6

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -