In Vitro Reconstitution of Autophagosome–Lysosome Fusion

J. Diao, L. Li, Y. Lai, Q. Zhong

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations


SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) proteins are a highly regulated class of membrane proteins lying in the center of membrane fusion. In conjunction with accessory proteins, SNAREs drive efficient merger of two distinct lipid bilayers into one interconnected structure. This chapter describes our fluorescence resonance energy transfer (FRET)-based proteoliposome fusion assays for the roles of various SNARE proteins, accessory proteins, and effects of different lipid compositions on membrane fusion involved in autophagy.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Number of pages12
StatePublished - 2017

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • ATG14
  • Accessory proteins
  • Autophagy
  • Fusion
  • Lipid
  • Lysosome
  • Single vesicle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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