In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

M. Gilmore-Hebert; R. W. Mercer; J. W. Schneider; E. J. Benz

In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

M. Gilmore-Hebert, R. W. Mercer, J. W. Schneider, E. J. Benz

Research output: Contribution to journal › Article › peer-review

Abstract

Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.

Original language	English (US)
Pages (from-to)	71-76
Number of pages	6
Journal	Progress in clinical and biological research
Volume	268 B
State	Published - 1988

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "In vitro expression of the alpha and beta subunits of the Na,K-ATPase.",

abstract = "Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.",

author = "M. Gilmore-Hebert and Mercer, {R. W.} and Schneider, {J. W.} and Benz, {E. J.}",

year = "1988",

language = "English (US)",

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pages = "71--76",

journal = "Progress in Clinical and Biological Research",

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publisher = "John Wiley and Sons Inc.",

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T1 - In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

AU - Gilmore-Hebert, M.

AU - Mercer, R. W.

AU - Schneider, J. W.

AU - Benz, E. J.

PY - 1988

Y1 - 1988

N2 - Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.

AB - Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.

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M3 - Article

C2 - 2851832

AN - SCOPUS:0024254139

SN - 0361-7742

VL - 268 B

SP - 71

EP - 76

JO - Progress in Clinical and Biological Research

JF - Progress in Clinical and Biological Research

ER -

In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

Abstract

ASJC Scopus subject areas

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