@article{dba646e34da44880bf31eabe8b0da6c5,
title = "Impaired function of the suprachiasmatic nucleus rescues the loss of body temperature homeostasis caused by time-restricted feeding",
abstract = "The suprachiasmatic nucleus (SCN) is the master circadian pacemaker that drives body temperature rhythm. Time-restricted feeding (TRF) has potential as a preventative or therapeutic approach against many diseases. The potential side effects of TRF remain unknown. Here we show that daily 4-h TRF treatment in mice can severely impair body temperature homeostasis and can result in lethality. Nearly half of the mice died at 21 °C, and all mice died at 18 °C during 4-h TRF. This effect was modulated by the circadian clock and was associated with severe hypothermia due to loss of body temperature homeostasis. Remarkably, disrupting the circadian clock by SCN lesions or constant light rescued lethality during TRF. Analysis of circadian gene expression in the dorsomedial hypothalamus (DMH) demonstrated that TRF reprograms rhythmic transcriptome in DMH and suppresses expression of genes, such as Ccr5 and Calcrl, which are involved in thermoregulation. Together, our results demonstrated a potent effect of TRF on body temperature homeostasis and the role of SCN function in this process. Our results further suggested that circadian arrhythmicity can have potential beneficial effects under certain stress conditions.",
keywords = "Body temperature, Circadian clock, Hypothermia, The dorsomedial hypothalamus, The suprachiasmatic nucleus, Time-restricted feeding",
author = "Zhihui Zhang and Qiaocheng Zhai and Yue Gu and Tao Zhang and Zhengyun Huang and Zhiwei Liu and Yi Liu and Ying Xu",
note = "Funding Information: We thank members of Cam-Su GRC for their assistance in animal facility and Xu{\textquoteright}s laboratory for discussion. We thank Yong Zhang, Xiaohan Wang, and Qingyu Wu for insightful comments. This work was supported by grants from the National Science Foundation of China ( 31630091 , 31230049 31600958 ), the Royal Society-Newton Advance Fellowship ( NA150373 ), and the Ministry of Science and Technology ( YFA0801100 ) to YX and by the National Institutes of Health ( 1R35GM118118 ), Cancer Prevention and Research Institute of Texas ( RP160268 ), and the Welch Foundation (I-1560) to YL. We also thank the Priority Academic Program Development of the Jiangsu Higher Education Institutes (PAPD) and National Center for International Research (2017B01012). Funding Information: We thank members of Cam-Su GRC for their assistance in animal facility and Xu's laboratory for discussion. We thank Yong Zhang, Xiaohan Wang, and Qingyu Wu for insightful comments. This work was supported by the National Natural Science Foundation of China (31630091, 31230049 31600958), the Royal Society-Newton Advance Fellowship (NA150373), and the Ministry of Science and Technology (YFA0801100) to YX and by the National Institutes of Health (1R35GM118118), Cancer Prevention and Research Institute of Texas (RP160268), and the Welch Foundation (I-1560) to YL. We also thank the Priority Academic Program Development of the Jiangsu Higher Education Institutes (PAPD) and National Center for International Research (2017B01012). Zhihui Zhang and Ying Xu conceived and designed the experiments; Zhihui Zhang, Qiaocheng Zhai, Tao Zhang and Zhengyun Huang performed experiments; Yue Gu analyzed RNA seq data; Zhiwei Liu designed equipment; Ying Xu and Yi Liu analyzed data and wrote the manuscript. Publisher Copyright: {\textcopyright} 2020 Science China Press",
year = "2020",
month = aug,
day = "15",
doi = "10.1016/j.scib.2020.03.025",
language = "English (US)",
volume = "65",
pages = "1268--1280",
journal = "Science Bulletin",
issn = "2095-9273",
publisher = "Springer Science + Business Media",
number = "15",
}