TY - JOUR
T1 - Immunologic mapping of the amino- and carboxy-termini of the turkey erythrocyte β-adrenergic receptor; Selective proteolysis of both domains
AU - Luxembourg, Alain
AU - Hekman, Mirko
AU - Ross, Elliott M.
N1 - Funding Information:
This work was Supported by NIH Grant GM30355 and R.A. Welch Foundation Grant 1982. Work carried out by M.H. in the laboratory of Prof. E.J.M. Helmreich (Würzburg) was supported by grants from the Deutsche Forschungsgemeinschaft Hc22/44-J and Project Grant Al to E.J.M.H. of Sonder-forschungsbereich 176 and by the Fonds der Chemischen Industrie e.V. M.H. wishes to acknowledge the interest and support of Prof. Ernst J.M. Helmreich. We thank Dr. Eric M. Parker for valuable discussion.
PY - 1991/5/20
Y1 - 1991/5/20
N2 - Peptide-directed antibodies were used to map the N- and C-termini of the turkey erythrocyte β-adrenergic receptor, the full length recombinant receptor expressed in Sf9 cells, and a mutant that terminates after residue 424 (T424). Both forms of the natural receptor (P40 and P50): were proteolytically clipped between residues 419 and 424, P40, but not P50, is also proteolyzed between residues 14 and 28. Truncation mutants, but not full length receptors, also display both large and small forms. The short form or T424 is formed by proteolysis after residue 14, but neither form is proteolyzed in the C-terminal region. The wild type recombinant receptor is not proteolyzed.
AB - Peptide-directed antibodies were used to map the N- and C-termini of the turkey erythrocyte β-adrenergic receptor, the full length recombinant receptor expressed in Sf9 cells, and a mutant that terminates after residue 424 (T424). Both forms of the natural receptor (P40 and P50): were proteolytically clipped between residues 419 and 424, P40, but not P50, is also proteolyzed between residues 14 and 28. Truncation mutants, but not full length receptors, also display both large and small forms. The short form or T424 is formed by proteolysis after residue 14, but neither form is proteolyzed in the C-terminal region. The wild type recombinant receptor is not proteolyzed.
KW - Antibody
KW - β-adrenergic receptor, Proteolysis: Immunogenic peptide
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U2 - 10.1016/0014-5793(91)80575-N
DO - 10.1016/0014-5793(91)80575-N
M3 - Article
C2 - 1645295
AN - SCOPUS:0025829439
SN - 0014-5793
VL - 283
SP - 155
EP - 158
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -