Immunohistochemical localization of sodium-dependent L-ascorbic acid transporter 1 protein in rat kidney

Ju Hyun Lee; Chang Seok Oh; Ga Hee Mun; Jae Hyup Kim; Yoon Hee Chung; Young Il Hwang; Dong Hoon Shin; Wang Jae Lee

doi:10.1007/s00418-006-0186-1

Immunohistochemical localization of sodium-dependent L-ascorbic acid transporter 1 protein in rat kidney

Ju Hyun Lee, Chang Seok Oh, Ga Hee Mun, Jae Hyup Kim, Yoon Hee Chung, Young Il Hwang, Dong Hoon Shin, Wang Jae Lee

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Recently, two l-ascorbic acid transporters were identified; sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2. The previous study suggested that SVCT protein might be present on the apical membrane in the straight segment (S3) of proximal tubule. In the present study, SVCT1 immunoreactivity (IR) was observed in the brush border of proximal straight tubules in the medullary ray of renal cortex and the outer stripe of outer medulla, while SVCT2 IR was not localized in any region of the kidney. Since the mechanism of VC reabsorption in the kidney has not been fully elucidated up to the present time, it is meaningful to demonstrate the exact cellular distribution of SVCT protein in the kidney.

Original language	English (US)
Pages (from-to)	491-494
Number of pages	4
Journal	Histochemistry and Cell Biology
Volume	126
Issue number	4
DOIs	https://doi.org/10.1007/s00418-006-0186-1
State	Published - Oct 2006
Externally published	Yes

Keywords

Immunohistochemistry
Kidney
L-ascorbic acid
Proximal tubule
Sodium-dependent vitamin C transporter

ASJC Scopus subject areas

Histology
Molecular Biology
Medical Laboratory Technology
Cell Biology

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10.1007/s00418-006-0186-1

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title = "Immunohistochemical localization of sodium-dependent L-ascorbic acid transporter 1 protein in rat kidney",

abstract = "Recently, two l-ascorbic acid transporters were identified; sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2. The previous study suggested that SVCT protein might be present on the apical membrane in the straight segment (S3) of proximal tubule. In the present study, SVCT1 immunoreactivity (IR) was observed in the brush border of proximal straight tubules in the medullary ray of renal cortex and the outer stripe of outer medulla, while SVCT2 IR was not localized in any region of the kidney. Since the mechanism of VC reabsorption in the kidney has not been fully elucidated up to the present time, it is meaningful to demonstrate the exact cellular distribution of SVCT protein in the kidney.",

keywords = "Immunohistochemistry, Kidney, L-ascorbic acid, Proximal tubule, Sodium-dependent vitamin C transporter",

author = "Lee, {Ju Hyun} and Oh, {Chang Seok} and Mun, {Ga Hee} and Kim, {Jae Hyup} and Chung, {Yoon Hee} and Hwang, {Young Il} and Shin, {Dong Hoon} and Lee, {Wang Jae}",

note = "Funding Information: This work was supported by BASIC RESEARCH GRANT (ROI-2005-000-10924-0) from the Korea Science and Engineering Foundation (KOSEF).",

year = "2006",

month = oct,

doi = "10.1007/s00418-006-0186-1",

language = "English (US)",

volume = "126",

pages = "491--494",

journal = "Histochemistry and Cell Biology",

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AU - Lee, Ju Hyun

AU - Oh, Chang Seok

AU - Mun, Ga Hee

AU - Kim, Jae Hyup

AU - Chung, Yoon Hee

AU - Hwang, Young Il

AU - Shin, Dong Hoon

AU - Lee, Wang Jae

N1 - Funding Information: This work was supported by BASIC RESEARCH GRANT (ROI-2005-000-10924-0) from the Korea Science and Engineering Foundation (KOSEF).

PY - 2006/10

Y1 - 2006/10

N2 - Recently, two l-ascorbic acid transporters were identified; sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2. The previous study suggested that SVCT protein might be present on the apical membrane in the straight segment (S3) of proximal tubule. In the present study, SVCT1 immunoreactivity (IR) was observed in the brush border of proximal straight tubules in the medullary ray of renal cortex and the outer stripe of outer medulla, while SVCT2 IR was not localized in any region of the kidney. Since the mechanism of VC reabsorption in the kidney has not been fully elucidated up to the present time, it is meaningful to demonstrate the exact cellular distribution of SVCT protein in the kidney.

AB - Recently, two l-ascorbic acid transporters were identified; sodium-dependent vitamin C transporter (SVCT) 1 and SVCT2. The previous study suggested that SVCT protein might be present on the apical membrane in the straight segment (S3) of proximal tubule. In the present study, SVCT1 immunoreactivity (IR) was observed in the brush border of proximal straight tubules in the medullary ray of renal cortex and the outer stripe of outer medulla, while SVCT2 IR was not localized in any region of the kidney. Since the mechanism of VC reabsorption in the kidney has not been fully elucidated up to the present time, it is meaningful to demonstrate the exact cellular distribution of SVCT protein in the kidney.

KW - Immunohistochemistry

KW - Kidney

KW - L-ascorbic acid

KW - Proximal tubule

KW - Sodium-dependent vitamin C transporter

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Immunohistochemical localization of sodium-dependent L-ascorbic acid transporter 1 protein in rat kidney

Abstract

Keywords

ASJC Scopus subject areas

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