Immunocytochemical characterization of secretory granule maturation in pancreatic a-cells

M. Ravazzola, A. Perrelet, Roger H Unger, L. Orci

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Antigenic sites related to glucagon and glucagon precursors were characterized by ultrastructural immunocytochemistry in secretory compartments of the pancreatic A-cell, namely Golgi cisternae, condensing granules in Golgi cisternae, coated immature secretory granules, and noncoated mature secretory granules. The C-terminal glucagon immunoreactivity was low in all these compartments except in the noncoated mature secretory granules. By contrast, N-terminal glucagon and glicentin immunoreactivities were high at the condensing granule stage and, respectively, increased (N-terminal) or decreased (glicentin) until the mature secretory granule stage. These data suggest that: 1) glucagon precursors and glucagon coexist at different concentrations in secretory compartments of the A-cell; 2) the condensing granules in Golgi cisternae and immature granules consist predominantly of glucagon precursors; and 3) the removal of the peptide masking the C-terminal of the glucagon molecule occurs between the coated and the mature granule stages.

Original languageEnglish (US)
Pages (from-to)481-485
Number of pages5
Issue number2
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Endocrinology


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