Immunoaffinity purification of protein complexes from mammalian cells

Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway, Joan W. Conaway

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations


In this chapter, we describe a purification scheme designed to isolate multisubunit protein complexes gently and quickly from crude extracts of mammalian cells using immunoaffinity purification of epitope tagged proteins and the multisubunit complexes with which they associate. As an example we describe isolation of the mammalian Mediator complex from HeLa S3 cells.

Original languageEnglish (US)
Title of host publicationGene Regulation
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages15
ISBN (Print)9781627032834
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Epitope tag
  • Immunoaffinity purification
  • Multisubunit protein complex
  • Stable cell line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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